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The complete genome sequence of Escherichia coli K-12 (1997)
| Venue: | Science |
| Citations: | 1128 - 39 self |
BibTeX
@ARTICLE{Blattner97thecomplete,
author = {Frederick R. Blattner and Guy Plunkett Iii and Craig A. Bloch and Nicole T. Perna and Valerie Burl and Monica Riley and Julio Collado-vides and Jeremy D. Glasner and Christopher K. Rode and George F. Mayhew and Jason Gregor and Nelson Wayne Davis and Heather A. Kirkpatrick and Michael A. Goeden and Debra J. Rose and Bob Mau and Ying Shao},
title = {The complete genome sequence of Escherichia coli K-12},
journal = {Science},
year = {1997},
pages = {1453--1461}
}
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Abstract
The 4,639,221–base pair sequence of Escherichia coli K-12 is presented. Of 4288 protein-coding genes annotated, 38 percent have no attributed function. Comparison with five other sequenced microbes reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident. The largest family of paralogous proteins contains 80 ABC transporters. The genome as a whole is strikingly organized with respect to the local direction of replication; guanines, oligo-nucleotides possibly related to replication and recombination, and most genes are so oriented. The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer. Because of its extraordinary position as a preferred model in biochemical genetics, molecular biology, and biotechnology, E. coli K-12 was the earliest organism to be suggested as a candidate for whole genome
