BibTeX
@MISC{_phosphoproteomeof,
author = {},
title = {Phosphoproteome of Embryonic Stem Cells},
year = {}
}
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Abstract
ison yielded almost no consensus, suggesting that the stem cell phenotype cannot be explained by transcriptional profile alone (14, 15). Clearly mechanisms operating at the posttranscriptional level may also be relevant. To date, proteomic analysis of embryonic stem cells has been limited. Guo et al. (16) used comparative two-dimensional gel electrophoresis (2DGE) to examine the differentiation of ESCs into neural cells under retinoic acid treatment and found 24 differentially expressed spots of which 12 were identified. Elliott et al. (17) produced a two-dimension gel electrophoresis map of proteins in the mouse ESC line R1 and identified 218 proteins. Recently Nagano et al. (18) used 2D LC-MS/MS to identify �1800 proteins from the ESC line E14-1. 35 of these proteins yielded mass spectra that were consistent with phosphorylation, and one phosphorylation
Keyphrases
embryonic stem cell two-dimension gel electrophoresis map expressed spot proteomic analysis esc line e14-1 neural cell posttranscriptional level transcriptional profile retinoic acid treatment lc-ms m mass spectrum comparative two-dimensional gel electrophoresis mouse esc line r1 stem cell phenotype cannot
