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The role of actin in spindle orientation changes during the Saccharomyces cerevisiae cell cycle (1999)
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| Venue: | J |
| Citations: | 19 - 2 self |
BibTeX
@ARTICLE{Theesfeld99therole,
author = {Ra L. Theesfeld and Javier E. Irazoqui and Kerry Bloom and Daniel J. Lew},
title = {The role of actin in spindle orientation changes during the Saccharomyces cerevisiae cell cycle},
journal = {J},
year = {1999}
}
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Abstract
Abstract. In the budding yeast Saccharomyces cerevisiae, the mitotic spindle must align along the motherbud axis to accurately partition the sister chromatids into daughter cells. Previous studies showed that spindle orientation required both astral microtubules and the actin cytoskeleton. We now report that maintenance of correct spindle orientation does not depend on F-actin during G2/M phase of the cell cycle. Depolymerization of F-actin using Latrunculin-A did not perturb spindle orientation after this stage. Even an early step in spindle orientation, the migration of the spindle pole body (SPB), became actin-independent if it was delayed until late in the cell cycle. Early in the cell cycle, both SPB migration and spindle orientation were very sensitive to perturbation of
Keyphrases
spindle orientation saccharomyces cerevisiae cell cycle spindle orientation change cell cycle budding yeast saccharomyces cerevisiae spb migration correct spindle orientation sister chromatid previous study early step mitotic spindle astral microtubule spindle pole body g2 phase motherbud axis actin cytoskeleton daughter cell
