BibTeX
@MISC{Pmsi_staphylococcusaureus,
author = {J. T. Pmsi and J. N. Baldwin and Margaret Sottile and L. Vidal},
title = {STAPHYLOCOCCUS AUREUS},
year = {}
}
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Abstract
THE PRODUCTION of coagulase is universally accepted as the most distinctive characteristic of Staphylococcus aureus. Furthermore, among the numerous extracellular proteins com-monly associated with staphylococcal virulence, coagulase seems to be the most stably associated. Recently we have developed a technique whereby it is readily possible to detect one colony that produces coagulase among 30 OOO coagulase-negative colonies by means of a pour-plate technique (parisi, Baldwin and Sottile, 1973). Coagulase-producing colonies in the agar can be detected by the dense zone of precipitated fibrin around them. This technique makes feasible certain types of genetic studies on production and regulation of coagulase, as well as studies of other aspects of its possible role in staphylococcal disease. MATERIALS AND METHODS Bacteriaf strains. A coagulase-negative mutant was obtained from strain 1-746 after exposure of the culture to 100 pg of N-methyl-N-nitro-N-nitrosoguanidine (Aldrich Chemical Co. Inc., Milwaukee, Wis.) per ml of 0. 1 ~ phosphate buffer, pH 7.0. Transduction experi-ments were done with the coagulase-negative mutant of strain 1-746 and with a lysogenic variant of this mutant strain. Both parent and mutant, before lysogenisation, were susceptible to lysis by phages 80 and 81 of the International Basic Set of staphylococcal phages. Upon lysogenisation, the coagulase-negative mutant was untypable. The S. aweus strains listed in the table were used in the curing experiments. All cultures were maintained on Difco Brain Heart Infusion
Keyphrases
staphylococcus aureus coagulase-negative mutant distinctive characteristic dense zone staphylococcal disease phosphate buffer lysogenic variant pour-plate technique technique whereby international basic set curing experiment staphylococcal virulence precipitated fibrin aweus strain ooo coagulase-negative colony genetic study mutant strain feasible certain type coagulase-producing colony transduction experi-ments possible role difco brain heart infusion staphylococcal phage aldrich chemical co method bacteriaf numerous extracellular protein
