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Cell Culture (2013)
BibTeX
@MISC{Wirth13cellculture,
author = {Peter J. Wirth and Stuart H. Yuspa and Snorri S. Thorgeirsson and Et Al and Cancer Res and Contact The Aacr Publications and Peter J. Wirth and Stuart H. Yuspa and Snorri S. Thorgeirsson and Henry Hennings},
title = {Cell Culture},
year = {2013}
}
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Abstract
Terminal differentiation can be induced in cultured basal cells by either increasing the Cai1 * level in the medium from 0.05 to 1.4 mM or by exposure to the tumor promoter 12-O-terradecanoylphorbol-13-acetate (TPA). If ( 'a2 * and TPA act by a common mechanism, then a common pattern of protein synthesis and/or phosphorylation would be expected. Computer-assisted analysis of radioactively labeled polypeptides sepa rated by two-dimensional-polyacrylamide gel electrophoresis was utilized to study protein synthesis and phosphorylation. Within l h of increasing the Ca2 * level in the medium, the synthesis of 57 polypeptides was altered by 2-fold or more. Similarly, exposure to TPA for l h affected the synthesis of 106 polypeptides. Sixteen polypeptides were affected by both ( 'a:+ and TPA; the synthesis of nine was increased and seven was decreased, with changes in the same direction for both effectors. By 4 h,
