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Robust processivity of myosin V under off-axis loads (2010)
BibTeX
@MISC{Oguchi10robustprocessivity,
author = {Yusuke Oguchi and Sergey V Mikhailenko and Takashi Ohki and Adrian O Olivares and Enrique M De La Cruz},
title = {Robust processivity of myosin V under off-axis loads},
year = {2010}
}
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Abstract
The dimeric motor myosin V transports organelles along actin filament tracks over long distances in cells. Myosin V is a smart ‘walker ’ that is able to swiftly adjust to variable ‘road conditions ’ to continue its processive movement across dense cellular environments. Coordination between the two heads via intramolecular load modulates biochemical kinetics and ensures highly efficient unidirectional motion. However, little is known about how load-induced regulation of the processive stepping occurs in vivo, where myosin V experiences significant off-axis loads applied in various directions. To reveal how myosin V remains processive in cells, we measured the effect of the off-axis loads, applied to individual actomyosin V bonds in a range of angles, on the coordination between the two heads and myosin V processive stepping. We found that myosin V remains highly processive under diagonal loads owing to asymmetrical ADP affinities and that the native 6IQ lever optimizes the subunit coordination, which indicates that myosin V is designed to be an intracellular transporter. © 2010 Nature America, Inc. All rights reserved. Myosin V transports cargos toward the barbed end of actin filaments in cells1,2, taking multiple ~36-nm steps by alternately swinging forward two lever arms in a ‘hand-overhand’ fashion3–7. Single-molecule experiments reveal that one ATP molecule is consumed for each step, which confirms the tight coupling
Keyphrases
off-axis load robust processivity actin filament track significant off-axis load lever arm variable road condition nature america intramolecular load asymmetrical adp affinity individual actomyosin bond diagonal load hand-overhand fashion3 subunit coordination myosin processive stepping efficient unidirectional motion actin filament long distance single-molecule experiment barbed end various direction dense cellular environment biochemical kinetics processive stepping occurs multiple 36-nm step dimeric motor myosin smart walker load-induced regulation atp molecule intracellular transporter processive movement tight coupling
