Prognostic Evaluation of DNA Index in HIV-HPV Co- Infected Women Cervical Samples Attending in Reference Centers for HIV-AIDS in Recife
Citations
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Citation Context ...dure prior sequencing using the Big Dye protocol (Applied Biosystems, SP, Brazil), in accordance with the manufacturer’s description. The chromatograms were firstly viewed using the Mega 5.0 software =-=[21]-=- in order to evaluate the quality of the sequence and comparisons were made with the reference sequences deposited in the GenBank database (http://www.ncbi. nlm.nih.gov), for viral typing identificati... |
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Citation Context ...in HIV-HPV Co-Infection PLOS ONE | www.plosone.org 5 August 2014 | Volume 9 | Issue 8 | e104801 concordance (k = 0.031), according to the kappa interpretation table proposed by Landis and Koch (1977) =-=[24]-=- (Table 4). Among the 33 women with progression to aneuploidy, 17 had oncotic cytology results from their samples at diagnosis and at the return consultation. Among these 17 women, 13 did not present ... |
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Citation Context ...presence of high-risk HPV contributes towards cervical lesions, especially CIN [2,28,29]. High-risk HPV types are closely associated with appearance and persistence of pre-neoplastic cervical lesions =-=[1,2,30]-=-, given that the viral proteins E6 and E7 bind to and inhibit the action of the human proteins p53 and pRB, which control the cell cycle. This culminates in establishment of chromosomal aberrations an... |
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The papillomavirus life cycle".
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Citation Context ...proteins involved in progression to the S phase of the cell cycle. The protein E6 supplements the function of the protein E7 by inactivating p53 and impeding the induction of apoptosis in these cells =-=[4,5]-=-. Prolongation of the S phase through the action of E6 and E7 may lead to chromosome instability and consequently to alteration of cell ploidy. These events are worsened by the phenomenon of viral int... |
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Fauquet C, Broker TR, Bernard HU, zur Hausen H (2004) Classification of papillomaviruses. Virology 324
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Citation Context ...ral typing identification. The viral isolates were classified in low-risk (types 6, 11, 61, 54, 62, 71, 72, 81, 84, 85, 86) or high-risk (types 16, 18, 31, 33, 45, 52, 53, 56, 58, 59, 66, 69, 70, 82) =-=[22]-=-, according to their known capacity in developing cancer. Determination of the DNA index The cervical cells were subjected to cellular lyses using Pharm Lyse lysing buffer (Becton Dickinson, New Jerse... |
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Human papillomavirus and cervical cancer.
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Citation Context ...y the phenomenon of viral integration, which may occur in situations of persistent infection by high-risk HPV, although the transformation phase may occur in stages that precede the viral integration =-=[6,7,8,9]-=-. The DNA index has been used to characterize cell ploidy in cases of endometrial cancer [10], liver cirrhosis [11] and acute lymphoid leukemia [12], and it is important in the prognosis for neoplasia... |
24 |
Detection of high-risk cervical intraepithelial neoplasia and cervical cancer by amplification of transcripts derived from integrated papillomavirus oncogenes. Cancer Res
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Citation Context ...y the phenomenon of viral integration, which may occur in situations of persistent infection by high-risk HPV, although the transformation phase may occur in stages that precede the viral integration =-=[6,7,8,9]-=-. The DNA index has been used to characterize cell ploidy in cases of endometrial cancer [10], liver cirrhosis [11] and acute lymphoid leukemia [12], and it is important in the prognosis for neoplasia... |
14 |
Biology of HPV
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Citation Context ... caused by the human papillomavirus (HPV) is a risk factor for the development of cervical cancer and, in patients coinfected with HIV, the cervical lesions are more severe and have a worse prognosis =-=[1,2,3]-=-. The role of HPV in the pathogenesis of cervical cancer involves interaction of the proteins E6 and E7 of high-risk HPV types with the tumorsuppressor nuclear proteins p53 and pRB, respectively. The ... |
14 |
Amon A (2008). Aneuploidy: cells losing their balance. Genetics 179
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Citation Context ... The DNA index has been used to characterize cell ploidy in cases of endometrial cancer [10], liver cirrhosis [11] and acute lymphoid leukemia [12], and it is important in the prognosis for neoplasia =-=[13]-=-. Some authors have also taken DNA index PLOS ONE | www.plosone.org 1 August 2014 | Volume 9 | Issue 8 | e104801 determinations to be a prospective prognostic parameter for cervical lesions caused by ... |
13 |
Kurman R, Moriarty A, O’Connor D, et al. (2002) The 2001 Bethesda System: terminology for the reporting results of cervical cytology
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Citation Context ...he molecular analysis on HPV, cervical scrapings were obtained using cytobrushes while performing the colposcopic examination. To classify the cytological findings, the Bethesda nomenclature was used =-=[17]-=-. The colposcopic findings were classified taking into consideration the classification criteria of the International Colposcopic Nomenclature of Rome [18]. In cases in which colposcopic alterations w... |
9 |
SB: Prognostic importance of blast cell DNA content in childhood acute lymphoblastic leukemia
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Citation Context ...r in stages that precede the viral integration [6,7,8,9]. The DNA index has been used to characterize cell ploidy in cases of endometrial cancer [10], liver cirrhosis [11] and acute lymphoid leukemia =-=[12]-=-, and it is important in the prognosis for neoplasia [13]. Some authors have also taken DNA index PLOS ONE | www.plosone.org 1 August 2014 | Volume 9 | Issue 8 | e104801 determinations to be a prospec... |
4 |
Bastert G, von Knebel Doeberitz M (2004) DNA aneuploidy and integration of human papillomavirus type 16 e6/ e7 oncogenes in intraepithelial neoplasia and invasive squamous cell carcinoma of the cervix uteri. Clin Cancer Res 10: 3059–3063
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Citation Context ...bind to and inhibit the action of the human proteins p53 and pRB, which control the cell cycle. This culminates in establishment of chromosomal aberrations and, consequently, appearance of aneuploidy =-=[5,31]-=-. Although this relationship is known, no association between aneuploidy and HPV infection was found in the present study, perhaps because of the preserved immunological state of the HIV-positive wome... |
4 |
Meys JF, Verstraelen H, Verhelst R, De Sutter P, Temmerman M, Vanden Broeck D: Association between bacterial vaginosis and cervical intraepithelial neoplasia: systematic review and meta-analysis. PloS one 2012
- Gillet
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Citation Context ...y (p = 0.001). It is known that chronic vaginal infections caused by anaerobic bacteria act as cofactors for CIN to appear, with consequent change of cell ploidy, independent of HIV and HPV infection =-=[32]-=-. In the present study, no data on associated bacteriosis and other infections of the female genital tract were evaluated, thereby limiting the understanding of this issue. HIV infection may in itself... |
4 |
Meintjes G (2011) Pathogenesis and prevention of immune reconstitution disease during antiretroviral therapy. Expert review of antiinfective therapy 9
- SD
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Citation Context ...at after use of antiretrovirals starts, there will be exacerbation of tissue inflammation caused by silent opportunistic cervical infections that are revealed through the reconstitutive immunotherapy =-=[34,35]-=-. An association between antiretroviral use and protection against emergence of CIN was recently demonstrated in HIV-positive women [36]. Our data suggest that the model for cervical lesion progressio... |
3 |
Vlahov D, Trimble CL, Timpson LA, et al. (2000) Cervical neoplasia and repeated positivity of human papillomavirus infection in human immunodeficiency virus-seropositive and -seronegative women. American j of epidemiology 151
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Citation Context ... caused by the human papillomavirus (HPV) is a risk factor for the development of cervical cancer and, in patients coinfected with HIV, the cervical lesions are more severe and have a worse prognosis =-=[1,2,3]-=-. The role of HPV in the pathogenesis of cervical cancer involves interaction of the proteins E6 and E7 of high-risk HPV types with the tumorsuppressor nuclear proteins p53 and pRB, respectively. The ... |
3 |
Atlas de diagnóstico diferencial em citologia ginecológica
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Citation Context ...tions were observed, a cervical biopsy was performed and the ensuing cervical tissue fragments were sent in 10% formol for histopathological analysis, taking into consideration the Schneider criteria =-=[19]-=-. The cervical scrapings were sent to the Molecular Biology Laboratory of the Pediatric Oncology Service of Instituto de Medicina Integral Prof. Fernando Figueira. The samples were subdivided: one fra... |
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Citation Context ... caused by the human papillomavirus (HPV) is a risk factor for the development of cervical cancer and, in patients coinfected with HIV, the cervical lesions are more severe and have a worse prognosis =-=[1,2,3]-=-. The role of HPV in the pathogenesis of cervical cancer involves interaction of the proteins E6 and E7 of high-risk HPV types with the tumorsuppressor nuclear proteins p53 and pRB, respectively. The ... |
2 |
Kreienberg R (2002) Molecular biology of cervical cancer and its precursors. Curr Womens Health Rep 2
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Citation Context ...y the phenomenon of viral integration, which may occur in situations of persistent infection by high-risk HPV, although the transformation phase may occur in stages that precede the viral integration =-=[6,7,8,9]-=-. The DNA index has been used to characterize cell ploidy in cases of endometrial cancer [10], liver cirrhosis [11] and acute lymphoid leukemia [12], and it is important in the prognosis for neoplasia... |
2 |
Vinokurova S (2009) Host factors in HPV-related carcinogenesis: cellular mechanisms controlling HPV infections
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Citation Context ...y the phenomenon of viral integration, which may occur in situations of persistent infection by high-risk HPV, although the transformation phase may occur in stages that precede the viral integration =-=[6,7,8,9]-=-. The DNA index has been used to characterize cell ploidy in cases of endometrial cancer [10], liver cirrhosis [11] and acute lymphoid leukemia [12], and it is important in the prognosis for neoplasia... |
2 |
DNA flow cytometry: a predictor of a high-risk group in cervical cancer. Cancer Detect Prev 21
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Citation Context ... have also taken DNA index PLOS ONE | www.plosone.org 1 August 2014 | Volume 9 | Issue 8 | e104801 determinations to be a prospective prognostic parameter for cervical lesions caused by HPV infection =-=[14,15]-=-, i.e. evolution comprising regression, progression or persistence of the cervical lesion [16]. There are no studies on women with HIV-HPV coinfection regarding tissue lesion progression using this te... |
2 |
von Knebel Doeberitz M, Bastert G 2001. Prospective clinical study comparing DNA flow cytometry and HPV typing as predictive tests for persistence and progression of CIN I/II. Cytometry 46
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Citation Context ...1 determinations to be a prospective prognostic parameter for cervical lesions caused by HPV infection [14,15], i.e. evolution comprising regression, progression or persistence of the cervical lesion =-=[16]-=-. There are no studies on women with HIV-HPV coinfection regarding tissue lesion progression using this technique; and therefore the aim of the present study was to evaluate the association between th... |
2 |
Soneja M (2011) HIV & immune reconstitution inflammatory syndrome (IRIS
- SK
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Citation Context ...at after use of antiretrovirals starts, there will be exacerbation of tissue inflammation caused by silent opportunistic cervical infections that are revealed through the reconstitutive immunotherapy =-=[34,35]-=-. An association between antiretroviral use and protection against emergence of CIN was recently demonstrated in HIV-positive women [36]. Our data suggest that the model for cervical lesion progressio... |
2 |
Highly active antiretroviral therapy and cervical dysplasia in HIV-infected women from South Africa.
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Citation Context ...s that are revealed through the reconstitutive immunotherapy [34,35]. An association between antiretroviral use and protection against emergence of CIN was recently demonstrated in HIV-positive women =-=[36]-=-. Our data suggest that the model for cervical lesion progression to cancer in HIV-positive women may be altered through the improvement of their immunological state that is provided by antiretroviral... |
1 |
Duerksen-Hughes PJ (2006) Modulation of apoptosis by human papillomavirus (HPV) oncoproteins. Arch Virol 151
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Citation Context ...proteins involved in progression to the S phase of the cell cycle. The protein E6 supplements the function of the protein E7 by inactivating p53 and impeding the induction of apoptosis in these cells =-=[4,5]-=-. Prolongation of the S phase through the action of E6 and E7 may lead to chromosome instability and consequently to alteration of cell ploidy. These events are worsened by the phenomenon of viral int... |
1 |
De Marzi P, Sassi I, Aletti G, et al. (2008) The role of DNA ploidy in postoperative management of stage I endometrial cancer. Ann Oncol 19
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Citation Context ...gh-risk HPV, although the transformation phase may occur in stages that precede the viral integration [6,7,8,9]. The DNA index has been used to characterize cell ploidy in cases of endometrial cancer =-=[10]-=-, liver cirrhosis [11] and acute lymphoid leukemia [12], and it is important in the prognosis for neoplasia [13]. Some authors have also taken DNA index PLOS ONE | www.plosone.org 1 August 2014 | Volu... |
1 |
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Citation Context ...the transformation phase may occur in stages that precede the viral integration [6,7,8,9]. The DNA index has been used to characterize cell ploidy in cases of endometrial cancer [10], liver cirrhosis =-=[11]-=- and acute lymphoid leukemia [12], and it is important in the prognosis for neoplasia [13]. Some authors have also taken DNA index PLOS ONE | www.plosone.org 1 August 2014 | Volume 9 | Issue 8 | e1048... |
1 |
Dotli S, Petrove M, et al. (2008) Adenocarcinoma of the uterine cervix: prognostic significance of clinicopathologic parameters, flow cytometry analysis and HPV infection. Acta Obstet Gynecol Scand l87: 366
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Citation Context ... have also taken DNA index PLOS ONE | www.plosone.org 1 August 2014 | Volume 9 | Issue 8 | e104801 determinations to be a prospective prognostic parameter for cervical lesions caused by HPV infection =-=[14,15]-=-, i.e. evolution comprising regression, progression or persistence of the cervical lesion [16]. There are no studies on women with HIV-HPV coinfection regarding tissue lesion progression using this te... |
1 |
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Citation Context ...amples was extracted using the proteinase K [Invitrogen, CA, USA] protocol and subjected to PCR using the degenerate primers MY09 and MY11 for amplification of the HPV L1 gene as previously described =-=[20]-=-. The PCR product was purified by conventional phenol-chloroform procedure prior sequencing using the Big Dye protocol (Applied Biosystems, SP, Brazil), in accordance with the manufacturer’s descripti... |
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Citation Context ... and it was used as the cut-off point to categorize this variable. It coincided with the median found in several studies of risk factors for HPV infection, thus enabling comparison with these studies =-=[23]-=-. The relationship with smoking was analyzed taking smokers and former smokers both separately and as a group, in comparison with nonsmokers. For the variable of alcohol abuse, the term ‘‘light drinke... |
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Citation Context ... of aneuploidy. It is possible that the income level was measuring another factor that was not evaluated in this study. Aneuploidy is frequently found in severe cervical lesions in HIV-negative women =-=[25,26]-=-, but this was not observed in the present study in relation to oncotic cytology, perhaps because of the low frequency of cases of altered cytology (12.0%). Oncotic cytological analysis is an importan... |
1 |
Bollmann M (2005) Aberrant, Highly Hyperdiploid Cells
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Citation Context ... of aneuploidy. It is possible that the income level was measuring another factor that was not evaluated in this study. Aneuploidy is frequently found in severe cervical lesions in HIV-negative women =-=[25,26]-=-, but this was not observed in the present study in relation to oncotic cytology, perhaps because of the low frequency of cases of altered cytology (12.0%). Oncotic cytological analysis is an importan... |
1 |
PM (2001) Biopsy correlates of abnormal cervical cytology classified using the Bethesda system. Gynecol Oncol 82: 516
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Citation Context ...low-cost test for screening for cervical lesions, but it is less specific that histological analysis for characterizing precancerous lesions, in which higher frequency of aneuploidy would be expected =-=[27]-=-. The presence of high-risk HPV contributes towards cervical lesions, especially CIN [2,28,29]. High-risk HPV types are closely associated with appearance and persistence of pre-neoplastic cervical le... |
1 |
Gonçalves CV, Gonçalves P, et al. (2010) Martinez AM. Human papillomavirus: prevalence and genotypes found among HIV-positive and negative women at a reference center in the far south of Brazil. Rev Soc Bras Med Trop 43
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Citation Context ...al analysis for characterizing precancerous lesions, in which higher frequency of aneuploidy would be expected [27]. The presence of high-risk HPV contributes towards cervical lesions, especially CIN =-=[2,28,29]-=-. High-risk HPV types are closely associated with appearance and persistence of pre-neoplastic cervical lesions [1,2,30], given that the viral proteins E6 and E7 bind to and inhibit the action of the ... |
1 |
Prevalence of human papillomavirus genotypes and associated cervical squamous intraepithelial lesions in HIV-infected women in Botswana
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Citation Context ...al analysis for characterizing precancerous lesions, in which higher frequency of aneuploidy would be expected [27]. The presence of high-risk HPV contributes towards cervical lesions, especially CIN =-=[2,28,29]-=-. High-risk HPV types are closely associated with appearance and persistence of pre-neoplastic cervical lesions [1,2,30], given that the viral proteins E6 and E7 bind to and inhibit the action of the ... |
1 |
DNA content can improve the detection and prognosis of carcinoma of the cervix. Biosci Trends 4
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Citation Context ...of the female genital tract were evaluated, thereby limiting the understanding of this issue. HIV infection may in itself increase the risks of cytological alterations and HPV infection independently =-=[33]-=-. In this regard, it is expected that after use of antiretrovirals starts, there will be exacerbation of tissue inflammation caused by silent opportunistic cervical infections that are revealed throug... |