Citation Context

... the aggregationsof the metallic nanoparticles have been reported previously in the literature. The development of biosignal detection methods with the use of plasmonic nanosensorsshas been mentioned =-=[26]-=-. The authors worked with thesextinction spectrum of both gold and silver nanoparticles,sdefining the extinction as the capacity to obscure or extinguish certain wavelength. In this sense a shift of t...

Citation Context

...ofsthe present work is to prepare functionalized gold na-snoparticles (FAuNPs) which could be able to detect asmembrane surface antigen, the 63 kDa glycoprotein na-smed as gp63 with protease activity =-=[19,20]-=-, which is ex-spressed on the surface of several Leishmania species,sincluds twelve different strains of Leishmania, includ-sing L. major, L. donovani, L. infantum, L. tropica, L. me-sxicana, L. amazo...

Citation Context

...pension, as well as to protein gp63, was evidenced. These tests were designed taking advantage ofsthe spectroscopic changes that took place due to the aggregation effect of the metallic nanoparticles =-=[22,24,25]-=-.sThe registered changes were used to expose the interaction of the FAuNPs complex with the gp63 protein. In allsour experiments we observed changes in the absorptionsspectra as a consequence of the F...

Citation Context

...on of the metallic nanoparticles generates aschange in their geometric form. When a different tridimensional form is obtained, a change in the plasmonicsresonance occurs causing a colorimetric change =-=[24,28,s29]-=-. This phenomenon is of interest for the present research project since our objective is to take advantage ofsthe changes in spectral properties for the development ofsa biotest for the early detectio...

Citation Context

...ntimonial treatment (N-methylglutamine) or liposomicsB amphotericine [15], indirect immunoperoxydase me-sthod [16], polymerase chain reaction [17], serology (ELI-sSA) and lymphoproliferative response =-=[18]-=-. In this way,sa sensible, cheap and reliable method for the diagnosis ofsLeishmaniosis occurrence is of great interest in order tosimprove the knowledge of the illness population preva-slence and hel...

Citation Context

...st gp63 Lei-sshmania genus protein. gp63: Glycoprotein 63 kDa fromsLeishmania genus. FAuNP: Functionalized gold nanoparticle). PS: Both promastigotes and FAuNP are not proportionally related in size.s=-=[27]-=-, one of the methods authors mention is the spectralschance due to metallic nanoparticles aggregation and thatsmethod can be used for direct detection of suspendedscells, viruses or microorganism rela...

Citation Context

...ia—sas its vector in the new world and Phlebotomous in thesold world. About 20 Leishmania species are responsiblesfor a wide range of clinical manifestations, some of themsare fatal if left untreated =-=[11]-=-. With an expanding endemicity, an estimated 350 million people at risk ands2,357,000 disability-adjusted life years lost, leishmaniosissis becoming a worldwide re-emerging public health pro-sblem. In...

Citation Context

...me of 25 zeptomoles. Also it has been developed a method to am-splify the wavelength shift observed from localized surface plasmon resonance (LSPR) bioassays in gold na-snoparticle-labeled antibodies =-=[10]-=-. This work involvessdetecting surface-bound analytes using gold nanoparticlesconjugated antibodies as a way to enhance SPR shifts forsCopyright © 2013 SciRes.sAJACsP. RODRÍGUEZsETsAL. 101 more sensit...

Citation Context

...s, metallic nanoparticles are been used as vehi-scle to detect specific biological targets. It has been usedsSPR to detect the hybridization of cDNA-coated goldsnanoparticles with a surface-bound RNA =-=[9]-=-. ThesescDNA-coated gold nanoparticles are used to detect ssDNAsdown to a concentration of 1 fM in a volume of 25 zeptomoles. Also it has been developed a method to am-splify the wavelength shift obse...

Citation Context

...3 Proteins1. IntroductionsNanotechnology appears to be a useful and promissorystool in biodiagnostics. The use of functionalized nano-sparticles is widely applied to detect presence of bio-smolecules =-=[1,2]-=-, cells and metallic ions [3-8]. Also, theyshave been used recently to study interactions betweensmolecules. These devices are frequently faster, simplersand cheaper than conventional detection method...

Citation Context

...3 Proteins1. IntroductionsNanotechnology appears to be a useful and promissorystool in biodiagnostics. The use of functionalized nano-sparticles is widely applied to detect presence of bio-smolecules =-=[1,2]-=-, cells and metallic ions [3-8]. Also, theyshave been used recently to study interactions betweensmolecules. These devices are frequently faster, simplersand cheaper than conventional detection method...

Citation Context

... nanoparticles coupled to lectin concanavalin A andsagglutinin have also been used in order to study thesspecificity of this device to three cell lines expressingsglycosyl complexes at their surfaces =-=[3]-=-. Parasite infec-stions are spread all over the planet, especially in tropicalsregions, making the population under poverty or withspoor conditions of life their most vulnerable target. Lei-sshmaniosi...

Citation Context

...utaneous, and diffuse cutaneous leishma-sniosis [12]. Over 70 species of Lutzomyia are suspectedsto be implicated in the transmission of eight Leishmaniasspecies known to infect dogs in South America =-=[13]-=-.sLutzomyia longipalpis is the most common vector forsvisceral leishmaniosis, and as many as 100 species of thessame genus are responsible for cutaneous and muco-cuta-sneous leishmaniosis. In Venezuel...

Citation Context

...ntry-sside are the most vulnerable segment of the population.sIn 1995 the Pan-American Health Organization reportedsthe existence of visceral, cutaneous, and muco-cutaneoussleishmaniosis in Venezuela =-=[14]-=-. The most affected areasin this country corresponds to the Andes region, wheresTáchira, Mérida and Trujillo states concentrate more thans60% of the infected population, with small farmers andsvillage...

Citation Context

..., indirect immuno-sfluorescence (using an anti-Leishmania IgG) confirmedsby western blotting, positive response to patients aftersantimonial treatment (N-methylglutamine) or liposomicsB amphotericine =-=[15]-=-, indirect immunoperoxydase me-sthod [16], polymerase chain reaction [17], serology (ELI-sSA) and lymphoproliferative response [18]. In this way,sa sensible, cheap and reliable method for the diagnosi...

Citation Context

... anti-Leishmania IgG) confirmedsby western blotting, positive response to patients aftersantimonial treatment (N-methylglutamine) or liposomicsB amphotericine [15], indirect immunoperoxydase me-sthod =-=[16]-=-, polymerase chain reaction [17], serology (ELI-sSA) and lymphoproliferative response [18]. In this way,sa sensible, cheap and reliable method for the diagnosis ofsLeishmaniosis occurrence is of great...

Citation Context

...by western blotting, positive response to patients aftersantimonial treatment (N-methylglutamine) or liposomicsB amphotericine [15], indirect immunoperoxydase me-sthod [16], polymerase chain reaction =-=[17]-=-, serology (ELI-sSA) and lymphoproliferative response [18]. In this way,sa sensible, cheap and reliable method for the diagnosis ofsLeishmaniosis occurrence is of great interest in order tosimprove th...

Citation Context

...ofsthe present work is to prepare functionalized gold na-snoparticles (FAuNPs) which could be able to detect asmembrane surface antigen, the 63 kDa glycoprotein na-smed as gp63 with protease activity =-=[19,20]-=-, which is ex-spressed on the surface of several Leishmania species,sincluds twelve different strains of Leishmania, includ-sing L. major, L. donovani, L. infantum, L. tropica, L. me-sxicana, L. amazo...

Citation Context

...pension, as well as to protein gp63, was evidenced. These tests were designed taking advantage ofsthe spectroscopic changes that took place due to the aggregation effect of the metallic nanoparticles =-=[22,24,25]-=-.sThe registered changes were used to expose the interaction of the FAuNPs complex with the gp63 protein. In allsour experiments we observed changes in the absorptionsspectra as a consequence of the F...