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Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science (1989)

by Choo QL, G Kuo, Weiner AJ, Overby LR, Bradley DW, M Houghton
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Hepatitis C virus (HCV) circulates as a population of different but closely related genomes: quasispecies nature of HCV genome distribution

by M Martell, J I Esteban, J Quer, J Genescà, A Weiner, R Esteban, J Gómez, Maria Martell, Juan I , 1992
"... Hepatitis C virus (HCV) circulates as a population of different but closely related genomes: quasispecies nature of HCV genome distribution. ..."
Abstract - Cited by 146 (10 self) - Add to MetaCart
Hepatitis C virus (HCV) circulates as a population of different but closely related genomes: quasispecies nature of HCV genome distribution.
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...ation of different but closely related genomes: quasispecies nature of HCV genome distribution. M Martell, J I Esteban, J Quer, J Genescà, A Weiner, R Esteban, J Guardia and J Gómez J. Virol. 1992, 66=-=(5)-=-:3225. Updated information and services can be found at: http://jvi.asm.org/content/66/5/3225 These include: Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» D...

Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex

by Denise Egger, Benno Wölk, Rainer Gosert, Hubert E. Blum, Darius Moradpour, Kurt Bienz, J. Virol, Denise Egger, Benno Wölk, Rainer Gosert, Leonardo Bianchi, Hubert E. Blum, Darius Moradpour, Kurt Bienz - J , 2002
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Abstract - Cited by 144 (10 self) - Add to MetaCart
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...g chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Despite the identification in 1989 of HCV as the most common etiologic agent of posttransfusion and sporadic non-A, non-B hepatitis =-=(15, 32)-=-, its replication cycle and pathogenesis are incompletely understood. Investigations have been hampered by the lack of a permissive cell culture system which would allow to investigate the full intrac...

Structure and organization of the hepatitis C virus genome isolated from human carriers

by A Takamizawa, C Mori, I Fuke, S Manabe, S Murakami, J Fujita, T Andoh, I Yoshida, H Okayama, J. Virol, A. Takamizawa, C. Mori, I. Fuke, S. Manabe, S. Murakami, J. Fujita, E. Onishi, T. Andoh, I. Yoshida, H. Okayama - J
"... isolated from human carriers. ..."
Abstract - Cited by 124 (1 self) - Add to MetaCart
isolated from human carriers.
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...-B hepatitis. Based on its physicochemical properties, the agent was suggested to be a virus (2, 3, 23), but virtually nothing was known about it until a recent breakthrough made by workers at Chiron =-=(5, 10)-=-. They cloned cDNAs corresponding to part of the genome of a virus isolated from a chimpanzee which had been infected with the blood of a patient with posttransfusion non-A, non-B hepatitis and which ...

Viral discovery and sequence recovery using DNA microarrays

by David Wang, Anatoly Urisman, Yu-tsueng Liu, Michael Springer, Thomas G. Ksiazek, Dean D. Erdman, Elaine R. Mardis, Matthew Hickenbotham, Vincent Magrini, James Eldred, J. Phillipe Latreille, Richard K. Wilson, Don Ganem, Joseph L. Derisi - PLoS Biol , 2003
"... Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. We describe herein a DNA microarray-based platform for novel virus identification ..."
Abstract - Cited by 82 (17 self) - Add to MetaCart
Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. We describe herein a DNA microarray-based platform for novel virus identification and characterization. Central to this approach was a DNA microarray designed to detect a wide range of known viruses as well as novel members of existing viral families; this microarray contained the most highly conserved 70mer sequences from every fully sequenced reference viral genome in GenBank. During an outbreak of severe acute respiratory syndrome (SARS) in March 2003, hybridization to this microarray revealed the presence of a previously uncharacterized coronavirus in a viral isolate cultivated from a SARS patient. To further characterize this new virus, approximately 1 kb of the unknown virus genome was cloned by physically recovering viral sequences hybridized to individual array elements. Sequencing of these fragments confirmed that the virus was indeed a new member of the coronavirus family. This combination of array hybridization followed by direct viral sequence recovery should prove to be a general strategy for the rapid identification and characterization of novel viruses and emerging infectious disease.

Persistent and transient replication of full-length hepatitis C virus genomes in cell culture

by Thomas Pietschmann, Volker Lohmann, Artur Kaul, Gabriele Rinck, Gabriel Rutter, Dennis Str, Ralf Bartenschlager, Thomas Pietschmann, Volker Lohmann, Artur Kaul, Nicole Krieger, Gabriele Rinck, Gabriel Rutter, Dennis Str, Ralf Bartenschlager - J , 2002
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...ors that are important for virus particle assembly and/or release. The hepatitis C virus (HCV) was identified as the causative agent for most posttransfusion and sporadic non-A, non-B hepatitis cases =-=(11, 45)-=-. According to recent estimates, about 170 million individuals worldwide are infected. One striking characteristic of HCV is its strong propensity to persist in the infected host, which often leads to...

Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J Virol. 1996;70:778-786. 2300 HAMAIA et al BLOOD, 15 OCTOBER 2001 z VOLUME 98, NUMBER 8 For personal use only.on September 11, 2016. by guest www.bloodjournal

by Jean Dubuisson, Charles, M. Rice - Hematology, 2021 L St, NW, Suite 900, Washington DC 20036. Blood (print ISSN 0006-4971, online ISSN 1528-0020), is
"... The hepatitis C virus (HCV) glycoproteins (E1 and E2) are released from the polyprotein by signal pepti-dase-mediated cleavage and interact to form a heterodimer. Since properly folded subunits are usually re-quired for specific recognition and stable oligomer formation, the rate of stable E1E2 comp ..."
Abstract - Cited by 65 (19 self) - Add to MetaCart
The hepatitis C virus (HCV) glycoproteins (E1 and E2) are released from the polyprotein by signal pepti-dase-mediated cleavage and interact to form a heterodimer. Since properly folded subunits are usually re-quired for specific recognition and stable oligomer formation, the rate of stable E1E2 complex formation, which is low, may be limited by the rate of HCV E1 and/or E2 folding. In this study, the folding of the HCV E1 and E2 glycoproteins was monitored by observing the kinetics of intramolecular disulfide bond formation. The association/dissociation of E1 and E2 with calnexin was also examined, since this molecular chaperone appears to play a major role in quality control via retention of incompletely folded or misfolded proteins in the endoplasmic reticulum. Our results indicate that the disulfide-dependent folding of E2 occurs rapidly and appears to be complete upon cleavage of the precursor E2-NS2. In contrast, folding of E1 is slow (>1 h), suggesting that this step may be rate limiting for E1E2 oligomerization. Both HCV glycoproteins associated rapidly with calnexin, but dissociation was slow, consistent with the slow folding and assembly of E1E2 glyco-protein complexes. These results suggest a role for prolonged association with calnexin in the folding and assembly of HCV glycoprotein heterodimer complexes. Hepatitis C virus (HCV), the major causative agent of non-A, non-B hepatitis (10, 40), is an enveloped virus contain-
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...gest a role for prolonged association with calnexin in the folding and assembly of HCV glycoprotein heterodimer complexes. Hepatitis C virus (HCV), the major causative agent of non-A, non-B hepatitis =-=(10, 40)-=-, is an enveloped virus containing a positive-strand RNA genome of approximately 9,500 nucleotides (10, 11). HCV (36, 47) has a similar genomic organization to the pestiviruses (12) and the flavivirus...

De novo initiation of RNA synthesis by the RNAdependent RNA polymerase (NS5B) of hepatitis C virus

by Guangxiang Luo, Robert K. Hamatake, Danielle M. Mathis, Jason Racela, Karen L. Rigat, Julie Lemm, Richard J, Guangxiang Luo, Robert K. Hamatake, Danielle M. Mathis, Jason Racela, Karen L. Rigat, Julie Lemm, Richard, J. Colonno - J , 2000
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Abstract - Cited by 63 (2 self) - Add to MetaCart
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...ously, HCV infection remains a major threat to the public health all over the world. HCV is an enveloped RNA virus containing a singlestranded positive-sense RNA genome approximately 9.5 kb in length =-=(14, 31, 56)-=-. The RNA genome consists of a 5�-untranslated region (5� UTR) of 341 nucleotides (12, 13), a large open reading frame (ORF) encoding a single polypeptide of 3,010 to 3,040 amino acids (14, 31, 56), a...

Involvement of endoplasmic reticulum chaperones in folding of hepatitis C virus glycoproteins

by Amélie Choukhi, Sophana Ung, Czeslaw Wychowski, Updated Information - J , 1998
"... These include: This article cites 59 articles, 30 of which can be accessed free at: ..."
Abstract - Cited by 52 (10 self) - Add to MetaCart
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...3,033 amino acids (39). Cleavages of this polyprotein are co- and posttranslational and generate at least 10 polypeptides including 2 glycoproteins, E1 and E2 (54). Since the molecular cloning of HCV =-=(4)-=-, characterization of its genomic organization and expression has progressed rapidly. However, despite this progress, data on the HCV life cycle remain scarce. This is due to the poor replication of H...

De novo initiation of RNA synthesis by hepatitis C virus nonstructural protein 5B polymerase

by Weidong Zhong, Annette S. Uss, Eric Ferrari, Johnson Y. N, Zhi Hong, Weidong Zhong, Annette S. Uss, Eric Ferrari, Johnson Y. N. Lau, Zhi Hong - Downloaded from http://jvi.asm.org/ on February 23, 2013 by PENN STATE UNIV , 2000
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...provides an opportunity to screen for inhibitors that specifically target the initiation step. Hepatitis C virus (HCV) is recognized as the causative agent for most cases of non-A and non-B hepatitis =-=(5, 12)-=-, with an estimated prevalence of 170 million worldwide (21). Upon first exposure to HCV, about 10 to 20% of infected individuals develop acute clinical hepatitis, while others appear to resolve the i...

Humoral immune response to hypervariable region 1 of the putative envelope glycoprotein (gp70) of hepatitis C virus

by Nobuyuki Kato, Hitomi Sekiya, Yuko Ootsuyama, L Takahide Nakazawa - Journal of Virology , 1993
"... We recently found that alterations of amino acids in hypervariable region 1 (HVR1) of the putative envelope glycoprotein (gp7O) of hepatitis C virus (HCV) occurred sequentially in the chronic phase of hepatitis at intervals of several months. This finding suggests that mutations in HVR1 are involved ..."
Abstract - Cited by 47 (6 self) - Add to MetaCart
We recently found that alterations of amino acids in hypervariable region 1 (HVR1) of the putative envelope glycoprotein (gp7O) of hepatitis C virus (HCV) occurred sequentially in the chronic phase of hepatitis at intervals of several months. This finding suggests that mutations in HVR1 are involved in the mechanism of persistent chronic HCV infection involving escape from the immunosurveillance system. To explore this possibility, we examined the humoral immune response to HVR1 with our assay system, in which immuno-precipitation was carried out with sera from patients by using an HVR1 (27-amino-acid) dihydrofolate reductase fusion protein synthesized by in vitro transcription and translation. Results showed that HVR1 contains a sequence-specific immunological epitope that induces the production of antibodies restricted to the specific viral isolate. Furthermore, analysis of the kinetics of the appearance of antibodies in two patients with chronic hepatitis, with whom successive alterations of amino acids of HVR1 have been observed, showed that the titers of anti-HVR1 antibodies usually reached maximal levels several months after the isolation of HCV having the specific sequence of HVR1. This observation suggests that anti-HVR1 antibodies are involved in the genetic drift of HVR1 (minor antigenic variation) by immunoselection. However, the coexistence of HVR1 as an antigen and its specific antibody was sometimes observed. The possibility that HVR1 acts as a neutralizing epitope is discussed.
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...bserved. The possibility that HVR1 acts as a neutralizing epitope is discussed. Hepatitis C virus (HCV) is the major etiological agent of posttransfusional non-A, non-B hepatitis throughout the world =-=(5, 27)-=- and is also considered to be a causative agent of chronic hepatitis, cirrhosis, and hepatocellular carcinoma in Japan (33, 35, 42). However, neither the mechanism(s) by which HCV causes these hepatic...

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