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...y to our understanding of plant organelle dynamics in living cells. Therefore, databases that compile image data of visualized organelles have been constructed (Cutler et al. 2000, Damme et al. 2004, =-=Brown et al. 2005-=-, Koroleva et al. 2005, Li et al. 2006, Mano et al. 2008), and are a useful ‘omics’ tool in systems biology analysis (Mano et al. 2009). The Plant Organelles Database (PODB) is a specialized database ...

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...c composition, gene expression and the prediction of protein localization (4–8). Recently, databases for individual organelles, including chloroplasts (9,10), mitochondria (11), vacuoles (12), nuclei =-=(13)-=-, peroxisomes (14,15) and cell walls (16), have been made available to provide information on organellar proteins identified in proteomic and/or sequence-based analyses. However, these databases are l...

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...c composition, gene expression and the prediction of protein localization (4–8). Recently, databases for individual organelles, including chloroplasts (9,10), mitochondria (11), vacuoles (12), nuclei =-=(13)-=-, peroxisomes (14,15) and cell walls (16), have been made available to provide information on organellar proteins identified in proteomic and/or sequence-based analyses. However, these databases are l...

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...more specific data on plantsnuclear proteins, such as AtNoPDB (http://bioinf.hutton.ac.uk/cgi-bin/atnopdb/home) thatsprovides a description of experimentally identified Arabidopsis nucleolar proteins =-=[93]-=-,sChromDB (http://www.chromdb.org/) which is an inventory of chromatin-associated proteinssfrom different species based on genomic and transcriptomic data [94], At-Dbome whichscollects in silico annot...

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... NPs. To undertake a comprehensive comparisonof theplant nucleolar proteomes based on a combined approach of alignment, structure and phylogeny an Arabidopsis nucleolar protein database was curetted (=-=Brown et al., 2005-=-). Similarly, comprehensive and well-annotated database of transcription factors may provide a useful resource to check annotations and to study gene regulatory pathways (Guo et al., 2005, 2008; Iida ...

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....g., units of mass). In addition, as shown in Table 2,sother databases gather image data that reveal protein localization and/or visualizedsorganelles (Fig. 1A, Cutler et al. 2000; Damme et al. 2004; =-=Brown et al. 2005-=-; Korolevaset al. 2005; Li et al. 2006; Mano et al. 2008). Although these databases deal with staticsimage data, some databases also collect movie data (Fig. 1B, Supplemental Movies 1, 2).sCompared wi...

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... it is intriguing that proteomic analysis of the Arabidopsis nucleolus identified 26 Sproteasome components that were not found in the corresponding analysis of human nucleolis(Andersen et al., 2002; =-=Brown et al., 2005-=-; Pendle et al., 2005).s141sFigure 5.5sN-terminally tagged RPL23aA and –B fusion proteins are degraded via a proteasome mediated pathway.sa and c Westernsblot using an anti-GFP antibody of Arabidopsis...