Massively parallel characterization of restriction endonucleases (2013)

by N Kamps-Hughes, A Quimby, Z Zhu, E A Johnson
Venue:Nucleic Acids Res
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Specificity of the ModA11, ModA12 and ModD1 epigenetic regulator N6-adenine DNA methyltransferases of Neisseria meningitidis

by Kate L. Seib, Freda E. -c. Jen, Aimee Tan, Adeana L. Scott, Ritesh Kumar, M. Power, Li-tzu Chen, Hsing-ju Wu, Andrew H. -j. Wang, Dorothea M. C. Hill, A. Luyten, Richard D. Morgan, Richard J. Roberts, Martin C. J. Maiden, Matthew Boitano, Tyson A. Clark, Jonas Korlach, Desirazu N. Rao , 2015
"... Phase variation (random ON/OFF switching) of gene expression is a common feature of host-adapted pathogenic bacteria. Phase variably expressed N6-adenine DNA methyltransferases (Mod) alter global methylation patterns resulting in changes in gene expression. These systems constitute phase vari-able r ..."
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Phase variation (random ON/OFF switching) of gene expression is a common feature of host-adapted pathogenic bacteria. Phase variably expressed N6-adenine DNA methyltransferases (Mod) alter global methylation patterns resulting in changes in gene expression. These systems constitute phase vari-able regulons called phasevarions. Neisseria menin-gitidis phasevarions regulate genes including viru-lence factors and vaccine candidates, and alter phe-notypes including antibiotic resistance. The target site recognized by these Type III N6-adenine DNA methyltransferases is not known. Single molecule, real-time (SMRT) methylome analysis was used to identify the recognition site for three key N. menin-gitidis methyltransferases: ModA11 (exemplified by M.NmeMC58I) (5′-CGYm6AG-3′), ModA12 (exempli-fied by M.Nme77I, M.Nme18I and M.Nme579II) (5′-