Results 1 - 10
of
17
The Crystal Structure of the SV40 T-Antigen Origin Binding Domain in Complex with DNA
"... DNA replication is initiated upon binding of ‘‘initiators’ ’ to origins of replication. In simian virus 40 (SV40), the core origin contains four pentanucleotide binding sites organized as pairs of inverted repeats. Here we describe the crystal structures of the origin binding domain (obd) of the SV4 ..."
Abstract
-
Cited by 8 (4 self)
- Add to MetaCart
DNA replication is initiated upon binding of ‘‘initiators’ ’ to origins of replication. In simian virus 40 (SV40), the core origin contains four pentanucleotide binding sites organized as pairs of inverted repeats. Here we describe the crystal structures of the origin binding domain (obd) of the SV40 large T-antigen (T-ag) both with and without a subfragment of origin-containing DNA. In the co-structure, two T-ag obds are oriented in a head-to-head fashion on the same face of the DNA, and each T-ag obd engages the major groove. Although the obds are very close to each other when bound to this DNA target, they do not contact one another. These data provide a high-resolution structural model that explains site-specific binding to the origin and suggests how these interactions help direct the oligomerization events that culminate in assembly of the helicase-active dodecameric complex of T-ag.
Flexible tethering of primase and DNA pol a in the eukaryotic primosome
- Nucleic Acids Res
, 2011
"... The Pol a/primase complex or primosome is the primase/polymerase complex that initiates nucleic acid synthesis during eukaryotic replication. Within the primosome, the primase synthesizes short RNA primers that undergo limited extension by Pol a. The resulting RNA–DNA primers are utilized by Pol d a ..."
Abstract
-
Cited by 4 (0 self)
- Add to MetaCart
(Show Context)
The Pol a/primase complex or primosome is the primase/polymerase complex that initiates nucleic acid synthesis during eukaryotic replication. Within the primosome, the primase synthesizes short RNA primers that undergo limited extension by Pol a. The resulting RNA–DNA primers are utilized by Pol d and Pol e for processive elongation on the lagging and leading strands, respectively. Despite its import-ance, the mechanism of RNA–DNA primer synthesis remains poorly understood. Here, we describe a structural model of the yeast primosome based on electron microscopy and functional studies. The 3D architecture of the primosome reveals an asymmet-ric, dumbbell-shaped particle. The catalytic centers of primase and Pol a reside in separate lobes of high relative mobility. The flexible tethering of the primo-some lobes increases the efficiency of primer trans-fer between primase and Pol a. The physical organization of the primosome suggests that a con-certed mechanism of primer hand-off between primase and Pol a would involve coordinated move-ments of the primosome lobes. The first three-dimensional map of the eukaryotic primosome at 25 A ̊ resolution provides an essential structural tem-plate for understanding initiation of eukaryotic replication.
Grid Computing in 3D-EM Image Processing using Xmipp
"... * These authors contributed equally to this work Image processing in three-dimensional electron microscopy (3D-EM) is characterized by large amounts of data, and voluminous computing requirements. Here, we report our first experience with grid computing in this area. We present an interface between ..."
Abstract
-
Cited by 1 (0 self)
- Add to MetaCart
(Show Context)
* These authors contributed equally to this work Image processing in three-dimensional electron microscopy (3D-EM) is characterized by large amounts of data, and voluminous computing requirements. Here, we report our first experience with grid computing in this area. We present an interface between grid computing middleware and our image processing package Xmipp. The efficacy of this approach was illustrated with an Xmipp application for estimation of the contrast transfer function. In addition, we report our experience with grid computing in the development of a novel image refinement algorithm based on maximum likelihood principles. Its extensive CPUrequirements might have seriously hampered the algorithm development, if not for the farreaching resources of grid computing. Our results suggest that electron microscopy image processing may be particularly well suited for grid computing. 1.
Contents lists available at ScienceDirect Journal of Structural Biology
"... journal homepage: www.elsevier.com/locate/yjsbi Automatic particle selection from electron micrographs using machine ..."
Abstract
- Add to MetaCart
(Show Context)
journal homepage: www.elsevier.com/locate/yjsbi Automatic particle selection from electron micrographs using machine
Flexible tethering of primase and DNA Pol a in the
, 2011
"... doi:10.1093/nar/gkr534 ..."
(Show Context)
Contents lists available at ScienceDirect Journal of Structural Biology
"... journal homepage: www.elsevier.com/locate/yjsbi A clustering approach to multireference alignment of single-particle projections ..."
Abstract
- Add to MetaCart
(Show Context)
journal homepage: www.elsevier.com/locate/yjsbi A clustering approach to multireference alignment of single-particle projections
Electron Microscopy
"... [Strategies for structural determination of biological specimens] ..."
(Show Context)
CONTENT ALERTS
, 1990
"... Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi. ..."
Abstract
- Add to MetaCart
(Show Context)
Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi.
CONTENT ALERTS
, 2008
"... This article cites 44 articles, 19 of which can be accessed free ..."
(Show Context)
