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Denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA. A new molecular approach to analyze the genetic diversity of mixed microbial communities
"... Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. ..."
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Cited by 460 (37 self)
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Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community.
Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA
- Appl. Environ
, 1997
"... Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. ..."
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Cited by 248 (7 self)
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Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.
Microbial biofilms: from ecology to molecular genetics
- Microbiol Mol Biol Rev
, 2000
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Cited by 177 (10 self)
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Updated information and services can be found at:
Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis
- J. Bacteriology
, 1996
"... Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected ..."
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Cited by 139 (6 self)
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Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected
Molecular microbial diversity in soils from eastern Amazonia: evidence for unusual microorganisms and microbial population shifts associated with deforestation
- Appl. Environ
, 1997
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Novel division level bacterial diversity in a Yellowstone hot
- J. Bacteriol
, 1998
"... A culture-independent molecular phylogenetic survey was carried out for the bacterial community in Obsidian Pool (OP), a Yellowstone National Park hot spring previously shown to contain remarkable archaeal ..."
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Cited by 121 (14 self)
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A culture-independent molecular phylogenetic survey was carried out for the bacterial community in Obsidian Pool (OP), a Yellowstone National Park hot spring previously shown to contain remarkable archaeal
Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation
- Appl. Environ. Microbiol. 64:3869
, 1998
"... These include: This article cites 34 articles, 27 of which can be accessed free at: ..."
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Cited by 110 (8 self)
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These include: This article cites 34 articles, 27 of which can be accessed free at:
Counting the uncountable: statistical approaches to estimating microbial diversity
- Appl. Environ
, 2001
"... This article cites 52 articles, 16 of which can be accessed free ..."
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Cited by 106 (0 self)
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This article cites 52 articles, 16 of which can be accessed free
2000a. Natural assemblages of marine proteobacteria and members of the Cytophaga Flavobacter cluster consuming low- and high-molecularweight dissolved organic
"... We used a method that combines microautoradiography with hybridization of fluorescent rRNA-targeted oligonucleotide probes to whole cells (MICRO-FISH) to test the hypothesis that the relative contributions of various phylogenetic groups to the utilization of dissolved organic matter (DOM) depend sol ..."
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Cited by 101 (9 self)
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We used a method that combines microautoradiography with hybridization of fluorescent rRNA-targeted oligonucleotide probes to whole cells (MICRO-FISH) to test the hypothesis that the relative contributions of various phylogenetic groups to the utilization of dissolved organic matter (DOM) depend solely on their relative abundance in the bacterial community. We found that utilization of even simple low-molecular-weight DOM components by bacteria differed across the major phylogenetic groups and often did not correlate with the relative abundance of these bacterial groups in estuarine and coastal environments. The Cytophaga-Flavobacter cluster was overrepresented in the portion of the assemblage consuming chitin, N-acetylglucosamine, and protein but was generally underrepresented in the assemblage consuming amino acids. The amino acid-consuming assemblage was usually dominated by the � subclass of the class Proteobacteria, although the representation of �-proteobacteria in the protein-consuming assemblages was about that expected from their relative abundance in the entire bacterial community. In our experiments, no phylogenetic group dominated the consumption of all DOM, suggesting that the participation of a diverse assemblage of bacteria is essential for the complete degradation of complex DOM in the oceans. These results also suggest that the role of aerobic heterotrophic bacteria in carbon cycling would be more accurately described by using three groups instead of the single bacterial compartment currently used in biogeochemical models.
Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorgansims
- Applications Environmental Microbiology
"... Recent progress in molecular microbial ecology has revealed that traditional culturing methods fail to represent the scope of microbial diversity in nature, since only a small proportion of viable microorganisms in a sample are recovered by culturing techniques. To develop methods to investigate the ..."
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Cited by 93 (9 self)
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Recent progress in molecular microbial ecology has revealed that traditional culturing methods fail to represent the scope of microbial diversity in nature, since only a small proportion of viable microorganisms in a sample are recovered by culturing techniques. To develop methods to investigate the full extent of microbial diversity, we used a bacterial artificial chromosome (BAC) vector to construct libraries of genomic DNA isolated directly from soil (termed metagenomic libraries). To date, we have constructed two such libraries, which contain more than 1 Gbp of DNA. Phylogenetic analysis of 16S rRNA gene sequences recovered from one of the libraries indicates that the BAC libraries contain DNA from a wide diversity of microbial phyla, including sequences from diverse taxa such as the low-G1C, gram-positive Acidobacterium, Cytophagales, and Proteobacteria. Initial screening of the libraries in Escherichia coli identified several clones that express heterologous genes from the inserts, confirming that the BAC vector can be used to maintain, express, and analyze environmental DNA. The phenotypes expressed by these clones include antibacterial, lipase, amylase, nuclease, and hemolytic activities. Metagenomic libraries are a powerful tool for exploring soil microbial diversity, providing access to the genetic information of uncultured soil microorganisms. Such libraries will be the basis of new initiatives to conduct genomic studies that link phylogenetic and functional information about the microbiota of environments dominated by microorganisms that are refractory to cultivation.
