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EFFECT OF THIDIAZURON ON IN VITRO REGENERAnON IN THREE BUCKWHEAT CULTIVARS
"... The frequency of shoot regeneration from callus in two diploid (cvs. Kora and Hruszowska) and one tetraploid (cv. Ernka) buckwheat cultivars was very low on BAP and IAA containing media but was substantially improved when a phenyl urea derivative-thidiazuron (TDZ) was substituted for BAP in the rege ..."
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The frequency of shoot regeneration from callus in two diploid (cvs. Kora and Hruszowska) and one tetraploid (cv. Ernka) buckwheat cultivars was very low on BAP and IAA containing media but was substantially improved when a phenyl urea derivative-thidiazuron (TDZ) was substituted for BAP in the regeneration medium. Thidiazuron alone had little effect on buckwheat regeneration and was effective only in combination with IAA. The observed shoot regeneration rates on IAA and TDZ-containing media were from ca. 30 to 50 % of shoot producing calli and from 0.53 to 0.98 shoots per callus fragment. Cv. Kora consistently yielded the most regenerated shoots, the regeneration rates of cvs. Hruszowska and Ernka were similar.
media and vessels
, 2002
"... vitro growth of Nepalese buckwheat embryos in response to culture ..."
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CALLUS FORMATION ANDPL ~ REGENERATION IN ANTHER CULTIJRES OF THREE BUCKWHEAT (FAGOPYRUM ESCULENTUMMoench.) CULTIVARS
"... Viable shoots were regenerated from anthers of one tetraploid (cv. Emka) and two diploid common buckwheat cultivars (cvs. Kora and Hruszowska) when cultures were maintained in a modified MS liquid medium. Callus development and shoot regeneration was consistently highest in the tetraploid cultivar a ..."
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Viable shoots were regenerated from anthers of one tetraploid (cv. Emka) and two diploid common buckwheat cultivars (cvs. Kora and Hruszowska) when cultures were maintained in a modified MS liquid medium. Callus development and shoot regeneration was consistently highest in the tetraploid cultivar and different in the two diploid cultivars. Viable shoots were produced by 10 % of the calli of the tetraploid and 4 % and 2.5 % of the calli of the diploids. The most prevalent class of the regenerants were tetraploids, regardless of cultivar. No haploids were found among regenerants derived from the diploid cultivars. Likewise, the tetraploid cultivar yielded no diploid regenerants.
Regeneration in vitro of three cultivars of buckwheat (Fagopyrum esculentum Moench.) as affected by medium compositron
, 1999
"... The frequency of shoot regeneration from callus in two diploid (cvs. Kora and Hruszowska) and one tetraploid (cv. Emka) buckwheat cultivars was very low on BAP and IAA containing media but was substan-tially improved when a phenyl urea derivative-thidiazuron (TDZ) was substituted for BAP in the rege ..."
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The frequency of shoot regeneration from callus in two diploid (cvs. Kora and Hruszowska) and one tetraploid (cv. Emka) buckwheat cultivars was very low on BAP and IAA containing media but was substan-tially improved when a phenyl urea derivative-thidiazuron (TDZ) was substituted for BAP in the regenera-tion medium. Thidiazuron alone had little effect on buckwheat regeneration and was effective only in combi-nation with IAA. The observed shoot regeneration rates on IAA and TDZ-containing media were from approximately 30 to 50 % of shoot producing calli and from 0.53 to 0.98 shoots per callus fragment. Cv. Kora was consistently the best yielder of regenerated shoots, while the regeneration rates of cvs. Hruszowska and Emka were similar.
