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Cloning and Functional Studies of a Splice Variant of CYP26B1 Expressed in Vascular Cells

by Ali Ateia Elmabsout, Ashok Kumawat, Olesya Krivospitskaya, Peder S. Olofsson, Leif A. Eriksson, Guro Valen
"... Background: All-trans retinoic acid (atRA) plays an essential role in the regulation of gene expression, cell growth and differentiation and is also important for normal cardiovascular development but may in turn be involved in cardiovascular diseases, i.e. atherosclerosis and restenosis. The cellul ..."
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Background: All-trans retinoic acid (atRA) plays an essential role in the regulation of gene expression, cell growth and differentiation and is also important for normal cardiovascular development but may in turn be involved in cardiovascular diseases, i.e. atherosclerosis and restenosis. The cellular atRA levels are under strict control involving several cytochromes P450 isoforms (CYPs). CYP26 may be the most important regulator of atRA catabolism in vascular cells. The present study describes the molecular cloning, characterization and function of atRA-induced expression of a spliced variant of the CYP26B1 gene. Methodology/Principal Findings: The coding region of the spliced CYP26B1 lacking exon 2 was amplified from cDNA synthesized from atRA-treated human aortic smooth muscle cells and sequenced. Both the spliced variant and full length CYP26B1 was found to be expressed in cultured human endothelial and smooth muscle cells, and in normal and atherosclerotic vessel. atRA induced both variants of CYP26B1 in cultured vascular cells. Furthermore, the levels of spliced mRNA transcript were 4.5 times higher in the atherosclerotic lesion compared to normal arteries and the expression in the

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by Benjamin D. Cosgrove, Linda G. Griffith, Alan J. Grodzinsky, Forest M. White, Linda G. Griffith , 2008
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Douglas A. Lauffenburger
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by Reza Zolfaghari, Qiuyan Chen, A. Catharine Ross, Zolfaghari R, Chen Q, Ross Ac. Dhrs , 2012
"... DHRS3, a retinal reductase, is differentially regulated by retinoic acid and lipopolysaccharide-induced inflammation in THP-1 cells and rat liver ..."
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DHRS3, a retinal reductase, is differentially regulated by retinoic acid and lipopolysaccharide-induced inflammation in THP-1 cells and rat liver
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Toxicology and Applied Pharmacology

by William Jaime Jo A, Xuefeng Ren B, Feixia Chu C, Maria Aleshin B, Henri Wintz A, Alma Burlingame C
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