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Detection of microsporidia by indirect immunofluorescence antibody test using polyclonal and monoclonal (1994)

by A M Aldras, J M Orenstein, D P Kotler, J A Shadduck, E S Didier
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Forecast Alert

by Keng Tee, Guihua Chen, Ling Lu, Wangxia Tong, Lin Gu, Chunhua Li, Teng Lu - IT Spending, Worldwide, 2008-2015, 4Q11 Update. 2012 http://www.gartner.com: http://www.gartner.com/it/content/1870000/1870018/january_10_it_spending_forecast_4q11_update_rgordon.p df?userId=57517080 , 2011
"... This article cites 42 articles, 15 of which can be accessed free ..."
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This article cites 42 articles, 15 of which can be accessed free

Production of Monoclonal Antibodies Directed against the Microsporidium Enterocytozoon bieneusi

by Enterocytozoon Bieneusi, Isabelle Accoceberry, Marc Thellier, Abderrahim Achbarou, Sylvestre Biligui, Martin Danis, Annick Datry, J. Clin Microbiol, Isabelle Accoceberry, Marc Thellier, Isabelle Desportes-livage, Abderrahim Achbarou, Sylvestre Biligui, Martin Danis, Annick Datry , 1999
"... These include: This article cites 33 articles, 19 of which can be accessed free at: ..."
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These include: This article cites 33 articles, 19 of which can be accessed free at:
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... France. Phone: 33 5-56 79 58 37. Fax: 33 5-56 79 58 79. E-mail: bernard.couprie@chu-aquitaine.fr. on the detection of the parasites’ spores in stools and other biological samples, have been proposed =-=(3, 16, 31, 39)-=-. Although PCR appears to be the most sensitive method (6, 9, 11, 23), immunological tools remain helpful for diagnosis and for epidemiological survey or experimental investigation. Specific monoclona...

Discrimination between Viable and Dead Encephalitozoon cuniculi (Microsporidian) Spores by Dual Staining with Sytox

by J. Clin Microbiol, L. C. Green, P. J. Leblanc, E. S. Didier, Calcofluor White Mr, Calcofluor White Mr, L. C. Green, P. J. Leblanc, E. S. Didier , 2000
"... This article cites 21 articles, 15 of which can be accessed free ..."
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This article cites 21 articles, 15 of which can be accessed free

Diagnosis of Intestinal Microsporidiosis by Examination of Stool and Duodenal Aspirate with Weber’s Modified

by Uvitex B Stains, P. C. Degirolami, C. R. Ezratty, G. Desai, A. Mccullough, D. Asmuth, C. Wanke, M. Federman , 1994
"... Severe, chronic diarrhea is a frequent complication of human immunodeficiency virus disease, and intestinal microsporidiosis is being recognized with increasing frequency in patients with AIDS. Noninvasive, cost-effective techniques are needed to optimize its diagnosis. Weber’s modified trichrome st ..."
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Severe, chronic diarrhea is a frequent complication of human immunodeficiency virus disease, and intestinal microsporidiosis is being recognized with increasing frequency in patients with AIDS. Noninvasive, cost-effective techniques are needed to optimize its diagnosis. Weber’s modified trichrome stain (MTS) and the fluorochrome Uvitex 2B stain were used to detect microsporidial spores in smears of stool and duodenal aspirate (DA) samples received from human immunodeficiency virus-infected patients for examination for ova and parasites. Of 305 samples (292 stool and 13 DA samples) from 140 patients examined by MTS, 83 samples from 26 (18.6%) of the patients were positive for microsporidia (23 patients diagnosed initially and 3 diagnosed upon review). A subset of the samples studied by MTS consisting of 108 smears of stool and DA specimens from 60 patients was examined by Uvitex 2B. All 44 samples positive by MTS were also positive by Uvitex 2B. In addition, seven specimens and three patients were initially detected as positive by Uvitex 2B only (all three patients were positive also by MTS upon review). Confirmation of the diagnosis was obtained for 24 of 26 smear-positive patients by duodenal biopsy and/or stool transmission electron microscopy. Of 114 patients with stained smears negative for microsporidia, 23 had duodenal biopsies which showed no microsporidia. For the 43 patients who underwent duodenal biopsy, the sensitivity of both the MTS and the Uvitex 2B methods compared with biopsy results was 100%. Of six patients with negative duodenal biopsies and positive stained
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.... hellem and murine polyclonal antisera to E. cuniculi and E. hellem were used to detect an Encephalitozoon sp. in tissue culture, E. bieneusi in a stool sample, and S. intestinalis in a urine sample =-=(1)-=-. More experience with the use of monoclonal and polyclonal antibodies and optimization of the technique so as to minimize background and cross-reactivity are needed. In this study, we evaluatedWeber’...

CONTENT ALERTS

by Angela Carville, Saul Tzipori, Inderpal Singh, Abhineet S. Sheoran, Quanshun Zhang, Virus-infected Macaques, Southborough Massachusetts , 2005
"... This article cites 29 articles, 20 of which can be accessed free ..."
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This article cites 29 articles, 20 of which can be accessed free
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...ological investigations of E. bieneusi infections in humans and in other mammals. With other mammalian microsporidia, specific polyclonal and monoclonal antibodies show strong cross-reactivity by IFA =-=(1, 15, 27, 29)-=-. None, however, cross-reacted with E. bieneusi spores, while the MAbs generated against E. bieneusi appear to reciprocate by being genus specific too, as they failed to react with other microsporidia...

These include:

by Kenny Rodriguez, See Profile, Kenny R. Rodriguez, Curt M. Horvath, Lgp Recognition Paramyxovirus V, Kenny R. Rodriguez, Curt M. Horvath , 2014
"... recognition by paramyxovirus V proteins: A ..."
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recognition by paramyxovirus V proteins: A
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...perienced eye. Polyclonal and polyclonal-absorbed antibodies for immunoassay may have lot-to-lot variation for wide diagnostic purposes, and the use of monoclonal antibodies that are species specific =-=(1, 3, 16, 20, 21, 26)-=- may not be helpful for identification of more than one microsporidian species. Monoclonal antibody 3B6 appears to be genus specific and recognizes Encephalitozoon spp. and related microsporidian spec...

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