Results 1 -
7 of
7
The cytosolic chaperonin CCT/TRiC and cancer cell proliferation
- PLoS ONE
"... The molecular chaperone CCT/TRiC plays a central role in maintaining cellular proteostasis as it mediates the folding of the major cytoskeletal proteins tubulins and actins. CCT/TRiC is also involved in the oncoprotein cyclin E, the Von Hippel-Lindau tumour suppressor protein, cyclin B and p21ras fo ..."
Abstract
-
Cited by 1 (0 self)
- Add to MetaCart
(Show Context)
The molecular chaperone CCT/TRiC plays a central role in maintaining cellular proteostasis as it mediates the folding of the major cytoskeletal proteins tubulins and actins. CCT/TRiC is also involved in the oncoprotein cyclin E, the Von Hippel-Lindau tumour suppressor protein, cyclin B and p21ras folding which strongly suggests that it is involved in cell proliferation and tumor genesis. To assess the involvement of CCT/TRiC in tumor genesis, we quantified its expression levels and activity in 18 cancer, one non-cancer human cell lines and a non-cancer human liver. We show that the expression levels of CCT/TRiC in cancer cell lines are higher than that in normal cells. However, CCT/TRiC activity does not always correlate with its expression levels. We therefore documented the expression levels of CCT/TRiC modulators and partners PhLP3, Hop/P60, prefoldin and Hsc/Hsp70. Our analysis reveals a functional interplay between molecular chaperones that might account for a precise modulation of CCT/TRiC activity in cell proliferation through changes in the cellular levels of prefoldin and/or Hsc/ p70 and CCT/TRiC client protein availability. Our observation and approaches bring novel insights in the role of CCT/TRiC-mediated protein folding machinery in cancer cell development.
Maximilians-Universität München Chaperonin-Catalyzed Rescue of Kinetically Trapped States in Protein Folding
"... I am grateful to Dr. Manajit Hayer-Hartl and Prof. Dr. F. Ulrich Hartl for giving me the opportunity to learn and interact with some of the best minds. I would like to take this opportunity to thank them for their encouragement, faith, continual support and giving me the freedom to learn from my mis ..."
Abstract
- Add to MetaCart
(Show Context)
I am grateful to Dr. Manajit Hayer-Hartl and Prof. Dr. F. Ulrich Hartl for giving me the opportunity to learn and interact with some of the best minds. I would like to take this opportunity to thank them for their encouragement, faith, continual support and giving me the freedom to learn from my mistakes. Their ideas and advice have been crucial for development of this project and me as a person. I would like to thank PD. Dr. Konstanze Winklhofer for being a co-referee of my thesis and my thesis advisory committee members Prof. Dr. Dr. Walter Neupert and Prof. Dr. Alexander Buchberger. I would also like to thank our very competent and helpful support system Andrea, Silke and Evelyn who were always there with kind words and solutions to all my administrative and other problems making my stay in Munich smooth. I am also grateful to Elisabeth, Bernd, Dirk, Nadine, and Emmanuel for all their logistical support and help. I am indebted to all my colleagues in the department of cellular biochemistry and specially office A-1 for making the working and learning a pleasurable experience. In particular, help and suggestions provided by Alex Hastie has been valuable to
unknown title
, 2014
"... TCP1 complex proteins interact with phosphorothioate oligonucleotides and can co-localize in oligonucleotide-induced nuclear bodies in mammalian cells ..."
Abstract
- Add to MetaCart
(Show Context)
TCP1 complex proteins interact with phosphorothioate oligonucleotides and can co-localize in oligonucleotide-induced nuclear bodies in mammalian cells
unknown title
, 2012
"... This Dissertation is brought to you for free and open access by BYU ScholarsArchive. It has been accepted for inclusion in All Theses and Dissertations by an authorized administrator of BYU ScholarsArchive. For more information, please contact scholarsarchive@byu.edu. ..."
Abstract
- Add to MetaCart
(Show Context)
This Dissertation is brought to you for free and open access by BYU ScholarsArchive. It has been accepted for inclusion in All Theses and Dissertations by an authorized administrator of BYU ScholarsArchive. For more information, please contact scholarsarchive@byu.edu.
unknown title
, 2014
"... TCP1 complex proteins interact with phosphorothioate oligonucleotides and can co-localize in oligonucleotide-induced nuclear bodies in mammalian cells ..."
Abstract
- Add to MetaCart
(Show Context)
TCP1 complex proteins interact with phosphorothioate oligonucleotides and can co-localize in oligonucleotide-induced nuclear bodies in mammalian cells
unknown title
, 2014
"... TCP1 complex proteins interact with phosphorothioate oligonucleotides and can co-localize in oligonucleotide-induced nuclear bodies in mammalian cells ..."
Abstract
- Add to MetaCart
(Show Context)
TCP1 complex proteins interact with phosphorothioate oligonucleotides and can co-localize in oligonucleotide-induced nuclear bodies in mammalian cells
der Ludwig-Maximilians-Universität München The Eukaryotic Chaperonin TRiC Domain-Wise Folding of Multi-Domain Proteins
"... I would like to thank Prof. Dr. F.-Ulrich Hartl for giving me the opportunity to work in his laboratory and for his optimism and his creative input during the entire course of my project. I would like to thank my supervisors Dr. Stephanie Etchells and Dr. Andreas Bracher. Many obstacles have been ov ..."
Abstract
- Add to MetaCart
(Show Context)
I would like to thank Prof. Dr. F.-Ulrich Hartl for giving me the opportunity to work in his laboratory and for his optimism and his creative input during the entire course of my project. I would like to thank my supervisors Dr. Stephanie Etchells and Dr. Andreas Bracher. Many obstacles have been overcome by their support. Their questions and ideas shaped the project significantly. I would like to thank Dr. Markus Stemp who had started the project and who introduced me into many of the necessary skills and techniques. I would like to thank my colleagues in the lab for creating a unique working atmosphere and for many shared moments that I will never forget. I would like to thank Dr. Birgitta Beatrix and Prof. Dr. Don Lamb for the discussions during the TAC meetings that provided helpful external input. I would like to thank the members of my PhD committee PD Dr. Dr. Konstanze Winklhofer, PD
