Abstract. In this paper we show that the twisted Ate pairing on elliptic curves can be generalized to hyperelliptic curves, we also give a series of variations of the hyperelliptic Ate and twisted Ate pairings. Using the hyperelliptic Ate pairing and twisted Ate pairing, we propose a new approach to speed up the Weil pairing computation, and obtain an interested result: For some hyperelliptic curves with high degree twist, using this approach to compute Weil pairing will be faster than Tate pairing, Ate pairing etc. all known pairings.