We present a novel method for the geometric alignment of autoradiographs of the brain. The method is based on finding the spatial mapping and the one-to-one correspondences (or homologies) between point features extracted from the images and rejecting non-homologies as outliers. In this way, we attempt to account for the local natural and artifactual differences between the autoradiograph slices. We have executed the resulting automated algorithm on a set of left prefrontal cortex autoradiograph slices, specifically demonstrated its ability to perform point outlier rejection, validated it using synthetically generated spatial mappings and provided a visual comparison against the well known iterated closest point (ICP) algorithm. Visualization of a stack of aligned left prefrontal cortex autoradiograph slices is also provided.

This manuscript tackles the registration of 2D biological images (histological sections or autoradiographs) to 2D images from the same or di#erent modalities (e.g., histology or MRI). The process of acquiring these images typically induces composite transformations that can be modeled as a number of rigid or a#ne local transformations embedded in an elastic one. We propose a registration approach closely derived from this model. Given a pair of input images, we first compute a dense similarity field between them with a block matching algorithm. A hierarchical clustering algorithm then automatically partitions this field into a number of classes from which we extract independent pairs of sub-images. Finally, the pairs of sub-images are, independently, a#nely registered and a hybrid a#ne/non-linear interpolation scheme is used to compose the output registered image. We investigate the behavior of our approach under a variety of conditions, and discuss examples using real biomedical images, including MRI, histology and cryosection data.

In this report, we present a new method of aligning histological sections. First a displacement field between the two images is computed by block matching. Then we estimate a rigid transformation based on the field. The process is integrated within a multi-scale scheme. We carefully study the problem of robustness and we propose several ideas to deal with intersection intensity differences and background artifacts. We demonstrate experimentally that we can reach a sub-voxel accuracy and we show some results on histological sections of a rat's brain and an endometrical adenocarcinoma.

Abstract. We present an image driven approach to the reconstruction of 3-D volumes from stacks of 2-D post-mortem sections (histology, cryoimaging, autoradiography or immunohistochemistry) in the absence of any external information. We note that a desirable quality of the reconstructed volume is the smoothness of its notable structures (e.g. the gray/white matter surfaces in brain images). Here we propose to use smoothness as a means to drive the reconstruction process itself. From an initial rigid pair-wise reconstruction of the input 2-D sections, we extract the boundaries of structures of interest. Those are then evolved under a mean curvature flow modified to constrain the flow within 2-D planes. Sparse displacement fields are then computed, independently for each slice, from the resulting flow. A variety of transformations, from globally rigid to arbitrarily flexible ones, can then be estimated from those fields and applied to the individual input 2-D sections to form a smooth volume. We detail our method and discuss preliminary results on both real histological data and synthetic examples. 1

affine registration of biological images for

Abstract. The recent development of 3-D medical imaging devices has given access to the 3-D imaging of in vivo tissues, from an anatomical (MR, CT) or even functional point of view (fMRI, PET, SPECT). However, the resolution of these images is still not sufficient to image anatomical or functional details, that can only be revealed by in vitro imaging (e.g. histology, autoradiography).The deep motivation of this work is the comparison of activations detected by fMRI series analysis to the ones that can be observed in autoradiographic images. The aim of the presented work is to fuse the autoradiographic data with the pre-mortem anatomical MR image, to facilitate the above-mentioned comparison. We show that this fusion can be achieved by using only simple global transformations (rigid and affine), yielding a very satisfactory result. 1