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Packaging of brome mosaic virus subgenomic RNA is functionally coupled to replication-dependent transcription and translation of coat protein
- J
, 2006
"... In Brome mosaic virus (BMV), genomic RNA1 (gB1) and RNA2 (gB2), encoding the replication factors, are packaged into two separate virions, whereas genomic RNA3 (gB3) and its subgenomic coat protein (CP) mRNA (sgB4) are copackaged into a third virion. In vitro assembly assays performed between a serie ..."
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In Brome mosaic virus (BMV), genomic RNA1 (gB1) and RNA2 (gB2), encoding the replication factors, are packaged into two separate virions, whereas genomic RNA3 (gB3) and its subgenomic coat protein (CP) mRNA (sgB4) are copackaged into a third virion. In vitro assembly assays performed between a series of deletion variants of sgB4 and wild-type (wt) CP subunits demonstrated that packaging of sgB4 is independent of sequences encoding the CP open reading frame. To confirm these observations in vivo and to unravel the mechanism of sgB4 copackaging, an Agrobacterium-mediated transient in vivo expres-sion system (P. Annamalai and A. L. N. Rao, Virology 338:96–111, 2005) that effectively uncouples replication from packaging was used. Cultures of agrotransformants, engineered to express sgB4 and CP subunits either transiently (sgB4Trans and CPTrans) or in replication-dependent transcription and trans-lation when complemented with gB1 and gB2 (sgB4Rep and CPRep), were mixed in all four pair-wise combinations and infiltrated to Nicotiana benthamiana leaves to systematically evaluate requirements regulating sgB4 packaging. The data revealed that (i) in the absence of replication, packaging was nonspecific, since transiently expressed CP subunits efficiently packaged ubiquitous cellular RNA as well as transiently expressed sgB4 and its deletion variants; (ii) induction of viral replication increased specificity of RNA packaging; and most importantly, (iii) efficient packaging of sgB4, reminiscent of the
Flock house virus induces apoptosis by depletion of Drosophila inhibitor-of-apoptosis protein DIAP1
- J
, 2008
"... This article cites 65 articles, 33 of which can be accessed free ..."
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This article cites 65 articles, 33 of which can be accessed free
Viral Capsid Formation By
, 2015
"... This Dissertation is brought to you for free and open access by the Theses and Dissertations at Research Showcase @ CMU. It has been accepted for inclusion in Dissertations by an authorized administrator of Research Showcase @ CMU. For more information, please contact research- ..."
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This Dissertation is brought to you for free and open access by the Theses and Dissertations at Research Showcase @ CMU. It has been accepted for inclusion in Dissertations by an authorized administrator of Research Showcase @ CMU. For more information, please contact research-
1 A Virus-like Particle that Elicits Cross-reactive Antibodies to the Conserved Stem of Influenza Virus Hemagglutinin
"... Running title: Antibodies to a conserved helix of influenza virus HA ..."
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Efficient Flock House Virus RNA Polymerase Synthesis in Drosophila Cells �
, 2007
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These include: This article cites 44 articles, 27 of which can be accessed free at:
Correspondence
, 2007
"... cerevisiae results in the spontaneous maturation of tetravirus procapsids in vivo ..."
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cerevisiae results in the spontaneous maturation of tetravirus procapsids in vivo
Viruses: Synchronized Coexpression of Functional Multigenome RNA Components of an Animal and a Plant Virus in Nicotiana benthamiana
, 2007
"... These include: This article cites 64 articles, 40 of which can be accessed free at: ..."
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These include: This article cites 64 articles, 40 of which can be accessed free at:
CONTENT ALERTS
"... This article cites 60 articles, 26 of which can be accessed free ..."
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Particles with Distinct RNA Packaging Characteristics in the Same Cell†
, 2006
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