Results 1 - 10
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22
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins
- Nucleic Acids Res
, 2006
"... DNA polymerase zeta (pol z) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in mutagenesis in vivo, here we report the fidelity of DNA synthesis in vitro by yeast pol z alone and with RFC, P ..."
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Cited by 14 (3 self)
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DNA polymerase zeta (pol z) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in mutagenesis in vivo, here we report the fidelity of DNA synthesis in vitro by yeast pol z alone and with RFC, PCNA and RPA. Overall, the accessory proteins have little effect on the fidelity of pol z. Pol z is relatively accurate for single base insertion/deletion errors. However, the average base substitution fidelity of pol z is substantially lower than that of homologous B family pols a, d and «. Pol z is particularly error prone for substitutions in specific sequence con-texts and generates multiple single base errors clustered in short patches at a rate that is unprece-dented in comparison with other polymerases. The unique error specificity of pol z in vitro is consistent with Pol z-dependent mutagenic specificity reported in vivo. This fact, combined with the high rate of single base substitution errors and complex muta-tions observed here, indicates that pol z contributes to mutagenesis in vivo not only by extending mismatches made by other polymerases, but also by directly generating its own mismatches and then extending them.
The efficiency and fidelity of 8-oxo-guanine bypass by DNA polymerases {delta} and {eta
- Nucleic Acids Res
, 2009
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Authors
, 2008
"... Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya ..."
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Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya
unknown title
, 2007
"... Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya ..."
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Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya
Open Access Publications
, 2008
"... The efficiency and fidelity of 8-oxo-guanine bypass by DNA polymerases delta and eta ..."
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The efficiency and fidelity of 8-oxo-guanine bypass by DNA polymerases delta and eta
Authors
"... Recombinant adeno-associated virus integration sites in murine liver after ornithine transcarbamylase gene correction ..."
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Recombinant adeno-associated virus integration sites in murine liver after ornithine transcarbamylase gene correction
unknown title
, 2007
"... Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya ..."
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Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya
unknown title
, 2008
"... mutant with reduced fidelity and increased translesion synthesis capacity ..."
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mutant with reduced fidelity and increased translesion synthesis capacity
RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella
, 2014
"... The eukaryotic RecA homologue Rad51 is a key fac-tor in homologous recombination and recombina-tional repair. Rad51-like proteins have been identi-fied in yeast (Rad55, Rad57 and Dmc1), plants and vertebrates (RAD51B, RAD51C, RAD51D, XRCC2, XRCC3 and DMC1). RAD51 and DMC1 are the strand-exchange pro ..."
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The eukaryotic RecA homologue Rad51 is a key fac-tor in homologous recombination and recombina-tional repair. Rad51-like proteins have been identi-fied in yeast (Rad55, Rad57 and Dmc1), plants and vertebrates (RAD51B, RAD51C, RAD51D, XRCC2, XRCC3 and DMC1). RAD51 and DMC1 are the strand-exchange proteins forming a nucleofilament for strand invasion, however, the function of the par-alogues in the process of homologous recombina-tion is less clear. In yeast the two Rad51 paralogues, Rad55 and Rad57, have been shown to be involved in somatic and meiotic HR and they are essential to the formation of the Rad51/DNA nucleofilament counter-balancing the anti-recombinase activity of the SRS2 helicase. Here, we examined the role of RAD51B in the model bryophyte Physcomitrella patens. Mu-tant analysis shows that RAD51B is essential for the maintenance of genome integrity, for resistance to DNA damaging agents and for gene targeting. Fur-thermore, we set up methods to investigate meio-sis in Physcomitrella and we demonstrate that the RAD51B protein is essential for meiotic homologous recombination. Finally, we show that all these func-tions are independent of the SRS2 anti-recombinase protein, which is in striking contrast to what is found in budding yeast where the RAD51 paralogues are fully dependent on the SRS2 anti-recombinase func-tion.
unknown title
, 2012
"... A four-subunit DNA polymerase f complex containing Pol d accessory subunits is essential for PCNA-mediated mutagenesis ..."
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A four-subunit DNA polymerase f complex containing Pol d accessory subunits is essential for PCNA-mediated mutagenesis
