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...orming aggregates [52]. However, this does not appear to be specific to AT missing the N-terminal extension, since the folding of the SPATE Pet was not significantly different from Pertactin in vitro =-=[53]-=-. In vitro folding is nevertheless very different from in vivo events, where secreted proteins might encounter chaperones in the periplasm. Indeed, two SPATE “stalled” mutants have recently been used ...

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...al structure in the passenger domains of Bordetella pertussis pertactin and Escherichia coli Pet are markedly more stable than the N-terminal portions of these passenger domains (Junker et al., 2006; =-=Renn and Clark, 2008-=-). These results led us to propose a model for AT OM secretion in which the free Fig. 1. AT OM secretion models, and design of stalled constructs. A. Three models for OM secretion of AT proteins: vect...