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...r annealing, oligonucleotides were radiolabelled by filling-in the ends using [a- 32 P]dCTP.Preparationofbacterialfull-lengthv-Mybprotein and binding experiments were performed as described previously=-=(28)-=-. Chromatin immunoprecipitation (ChIP) Approximately 10 8 BM2 or HD11 cells were incubated in 1/10 crosslinking solution containing 0.1 M NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Tris–HCl, pH 8.0, and 11% ...

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...eled by filling in the ends using a 32 P-dCTP and Klenow polymerase. Bacterial v-Myb, using the expression vector pVM2028 and pVM2101, was prepared and binding experiments were performed as described =-=(30)-=-. pVM2028 encodes the v-Myb protein of AMV and has been described (31). pVM2101 is a derivative of pVM2028 generated by replacing the v-myb coding region between the NcoI and SalI sites of v-myb with ...

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...abeled by filling in the ends using a32P-dCTP and Klenow polymerase. Bacterial v-Myb, using the expression vector pVM2028 and pVM2101, was prepared and binding experiments were performed as described =-=(30)-=-. pVM2028 encodes the v-Myb protein of AMV and has been described (31). pVM2101 is a derivative of pVM2028 generated by replacing the v-myb coding region between the NcoI and SalI sites of v-myb with ...

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...abeled by filling in the ends using a32P-dCTP and Klenow polymerase. Bacterial v-Myb, using the expression vector pVM2028 and pVM2101, was prepared and binding experiments were performed as described =-=(30)-=-. pVM2028 encodes the v-Myb protein of AMV and has been described (31). pVM2101 is a derivative of pVM2028 generated by replacing the v-myb coding region between the NcoI and SalI sites of v-myb with ...

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...abeled by filling in the ends using a32P-dCTP and Klenow polymerase. Bacterial v-Myb, using the expression vector pVM2028 and pVM2101, was prepared and binding experiments were performed as described =-=(30)-=-. pVM2028 encodes the v-Myb protein of AMV and has been described (31). pVM2101 is a derivative of pVM2028 generated by replacing the v-myb coding region between the NcoI and SalI sites of v-myb with ...

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...abeled by filling in the ends using a32P-dCTP and Klenow polymerase. Bacterial v-Myb, using the expression vector pVM2028 and pVM2101, was prepared and binding experiments were performed as described =-=(30)-=-. pVM2028 encodes the v-Myb protein of AMV and has been described (31). pVM2101 is a derivative of pVM2028 generated by replacing the v-myb coding region between the NcoI and SalI sites of v-myb with ...

Citation Context

...abeled by filling in the ends using a32P-dCTP and Klenow polymerase. Bacterial v-Myb, using the expression vector pVM2028 and pVM2101, was prepared and binding experiments were performed as described =-=(30)-=-. pVM2028 encodes the v-Myb protein of AMV and has been described (31). pVM2101 is a derivative of pVM2028 generated by replacing the v-myb coding region between the NcoI and SalI sites of v-myb with ...

Citation Context

...abeled by filling in the ends using a32P-dCTP and Klenow polymerase. Bacterial v-Myb, using the expression vector pVM2028 and pVM2101, was prepared and binding experiments were performed as described =-=(30)-=-. pVM2028 encodes the v-Myb protein of AMV and has been described (31). pVM2101 is a derivative of pVM2028 generated by replacing the v-myb coding region between the NcoI and SalI sites of v-myb with ...