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5,256
A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res
"... Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as ..."
Abstract
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Cited by 1088 (4 self)
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Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable
Model-Based Analysis of Oligonucleotide Arrays: Model Validation, Design Issues and Standard Error Application
, 2001
"... Background: A model-based analysis of oligonucleotide expression arrays we developed previously uses a probe-sensitivity index to capture the response characteristic of a specific probe pair and calculates model-based expression indexes (MBEI). MBEI has standard error attached to it as a measure of ..."
Abstract
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Cited by 775 (28 self)
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of accuracy. Here we investigate the stability of the probe-sensitivity index across different tissue types, the reproducibility of results in replicate experiments, and the use of MBEI in perfect match (PM)-only arrays. Results: Probe-sensitivity indexes are stable across tissue types. The target gene
SURVEY AND SUMMARY Target mRNA inhibition by oligonucleotide drugs in man
, 2012
"... Oligonucleotide delivery in vivo is commonly seen as the principal hurdle to the successful develop-ment of oligonucleotide drugs. In an analysis of 26 oligonucleotide drugs recently evaluated in late-stage clinical trials we found that to date at least half have demonstrated suppression of the targ ..."
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of the target mRNA and/or protein levels in the relevant cell types in man, including those present in liver, muscle, bone marrow, lung, blood and solid tumors. Overall, this strongly implies that the drugs are being delivered to the appropriate disease tissues. Strikingly we also found that the majority
Silencing efficacy prediction: a retrospective study on target mRNA features
"... Post-transcriptional gene silencing is a widely used method to suppress gene expression. Unfortunately only a portion of siRNAs do successfully reduce gene expression. Target mRNA secondary structures and siRNA-mRNA thermodynamic features are believed to contribute to the silencing activity. However ..."
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Post-transcriptional gene silencing is a widely used method to suppress gene expression. Unfortunately only a portion of siRNAs do successfully reduce gene expression. Target mRNA secondary structures and siRNA-mRNA thermodynamic features are believed to contribute to the silencing activity
Ratio-Based Decisions and the Quantitative Analysis of cDNA Microarray Images
, 1997
"... Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation ..."
Abstract
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Cited by 366 (28 self)
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Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image
Review Article Targeting mRNA for Alzheimer’s and Related Dementias
"... which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Brain deposition of the amyloid beta-protein (A ..."
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which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Brain deposition of the amyloid beta-protein (A
REPORT Target mRNA abundance dilutes microRNA and siRNA activity
"... Post-transcriptional regulation by microRNAs and siRNAs depends not only on characteristics of individual binding sites in target mRNA molecules, but also on system-level properties such as overall molecular concentrations. We hypothesize that an intracellular pool of microRNAs/siRNAs faced with a l ..."
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Post-transcriptional regulation by microRNAs and siRNAs depends not only on characteristics of individual binding sites in target mRNA molecules, but also on system-level properties such as overall molecular concentrations. We hypothesize that an intracellular pool of microRNAs/siRNAs faced with a
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