We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation

Modeling and simulation of the main metabolism in Escherichia coli and its several single-gene knockout mutants with experimental verification

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have been devised to survey gene functions en masse either computationally (Marcotte et al., 1999) or experimentally; among these, highly parallel assays of

There are many sources of systematic variation in cDNA microarray experiments which affect the measured gene expression levels (e.g. differences in labeling efficiency between the two fluorescent dyes). The term normalization refers to the process of removing such variation. A constant adjustment

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PBAD promoter. arabinoseexpression by vectors containing the Tight regulation, modulation, and high-level

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In order to understand the functioning of organisms on the molecular level, we need to know which genes are expressed, when and where in the organism, and to which extent. The regulation of gene expression is achieved through genetic regulatory systems structured by networks of interactions between