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3,765
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol 2:2006.0008
, 2006
"... We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosom ..."
Abstract
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Cited by 714 (7 self)
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We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation
Open Access
"... Modeling and simulation of the main metabolism in Escherichia coli and its several single-gene knockout mutants with experimental verification ..."
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Modeling and simulation of the main metabolism in Escherichia coli and its several single-gene knockout mutants with experimental verification
Functional discovery via a compendium of expression profiles.
- Cell,
, 2000
"... provided that the cellular transcriptional response to frames encode proteins required for sterol metabodisruption of different steps in the same pathway is lism, cell wall function, mitochondrial respiration, or similar, and that there are sufficiently unique transcripprotein synthesis. We also sh ..."
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Cited by 547 (9 self)
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show that the compendium tional responses to the perturbation of most cellular can be used to characterize pharmacological perturpathways, systematic characterization of novel mutants bations by identifying a novel target of the commonly could be carried out with a single genome-wide expresused drug
Elucidating the essentiality of essential genes in Escherichia coli K-12
, 2006
"... Recently, 303 essential gene candidates of Escherichia coli K-12 have been obtained from the construction of in-frame, single-gene knockout mutants by the collaboration of Dr. Hirotada Mori, NAIST, Japan. Elucidating the essentiality of these essential genes is a key to understand the system-level o ..."
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Recently, 303 essential gene candidates of Escherichia coli K-12 have been obtained from the construction of in-frame, single-gene knockout mutants by the collaboration of Dr. Hirotada Mori, NAIST, Japan. Elucidating the essentiality of these essential genes is a key to understand the system
Generation of a Complete Single-Gene Knockout Bacterial Artificial Chromosome Library of Cowpox Virus and Identification of Its Essential Genes
"... Cowpox virus (CPXV) belongs to the genusOrthopoxvirus in the Poxviridae family. It infects a broad range of vertebrates and can cause zoonotic infections. CPXV has the largest genome among the orthopoxviruses and is therefore considered to have the most complete set of genes of all members of the ge ..."
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of the genus. Since CPXV has also become amodel for studying poxvirus genetics and pathogenesis, we created and characterized a complete set of single gene knockout bacterial artificial chromosome (BAC) clones of the CPXV strain Brighton Red. These mutants allow a systematic assessment of the contribution
H: Genome-wide screening with hydroxyurea reveals a link between nonessential ribosomal proteins and reactive oxygen species production
- J Bacteriol
, 2013
"... We performed a screening of hydroxyurea (HU)-sensitive mutants using a single-gene-deletion mutant collection in Escherichia coli. HU inhibits ribonucleotide reductase (RNR), which leads to arrest of the replication fork. Surprisingly, the wild-type was less resistant to HU than the average for the ..."
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Cited by 2 (0 self)
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-resistant phenotype. These mutants increased the copy number of the p15A-based plasmid and exhibited reduced basal levels of SOS response. The data suggest that nonessential pro-teins indirectly affect the DNA-damaging process. The Keio Collection is a complete set of precisely defined, non-essential, single-gene
Research Article Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei
"... Copyright © 2014 Suat Moi Puah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Gram-negative saprophyte Burkholderia pseud ..."
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with an additional gene BPSL3147 that had been identified by other investigators. Through a reverse genetic approach, single-gene knockout mutants were successfully constructed by using site-specific insertion mutagenesis and were confirmed by PCR. BPSL2033::Km and BURPS1710A 1419::Kmmutants showed reduced rates
Plant residue chemistry impacts soil processes and microbial community structure: a study with Arabidopsis thaliana cell wall mutants.
- Appl. Soil Ecol.
, 2012
"... a b s t r a c t The C:N ratio and concentrations of resistant compounds such as lignin in plant residues influences their biodegradation by microbial communities in soil. Soil texture is expected to modulate biodegradation rates through physical protection of residues and feedback on microbial comm ..."
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Cited by 1 (1 self)
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. thaliana wild ecotypes and single gene knockout mutants of cinnamoyl-CoA reductase 1 (CCR1) and production of anthocyanin pigment 1 (PAP1/MYB75). Ground stem and root residues from A. thaliana lines were then mixed separately with soil (clay loam or sandy loam) and incubated for 63 d at 25 • C to evaluate
Random Phenotypic Variation of Yeast (Saccharomyces cerevisiae) Single-Gene Knockouts Fits a Double Pareto- Lognormal Distribution
"... Background: Distributed robustness is thought to influence the buffering of random phenotypic variation through the scale-free topology of gene regulatory, metabolic, and protein-protein interaction networks. If this hypothesis is true, then the phenotypic response to the perturbation of particular ..."
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. It consists of a mixture of a lognormal body and upper and lower power-law tails. Objective and Methods: If our assumptions are true, the DPLN distribution should provide a better fit to random phenotypic variation in a large series of single-gene knockout lines than other skewed or symmetrical distributions
CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in
"... ABSTRACT Trypanosoma cruzi is a protozoan parasite of humans and animals, affecting 10 to 20 million people and innumerable animals, primarily in the Americas. Despite being the largest cause of infection-induced heart disease worldwide, even among the neglected tropical diseases (NTDs) T. cruzi is ..."
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for the genetic engineer-ing of T. cruzi, demonstrating rapid and efficient knockout of multiple endogenous genes, including essential genes. We ob-served that in the absence of a template, repair of the Cas9-induced double-stranded breaks (DSBs) in T. cruzi occurs exclusively by microhomology-mediated end
Results 1 - 10
of
3,765
