Summary In spring, the lowest temperature during freezing that can be survived by wood frogs (Rana sylvatica) from southern Ohio is approximately Ϫ3˚C. We investigated whether the thermal limit of freeze tolerance in these frogs is regulated by tissue levels of glucose, a putative cryoprotectant

TRAQ™) peptide mapping to assess variation in the abundance of hepatic proteins in summer- and winter-acclimatized wood frogs (Rana sylvatica), a northerly-distributed species that tolerates extreme dehydration and tissue freezing during hibernation. Thirty-three unique proteins exhibited strong seasonal

. We adopted this approach in a study of plastic morphological traits in larvae of the wood frog, Rana sylvatica. First we reared tadpoles in arti®cial ponds for 18 days, in either the presence or absence of Anax dragon¯y larvae (con®ned within cages to prevent them from killing the tadpoles

Summary. Wood frogs ( Ranu syluatica) were frozen to-2.5"C under f ive d is t inct cool ing regimes to invest igate the effect of cooling rate on survival. Frogs survived f reez ing whcn coo led a t 0.16 'C.h l o r- 0.18 'C.h 1,

Many amphibians lay their eggs in gelatinous masses up to 10–20 cm in diameter, posing problems for diffusive oxygen delivery. Oxygen may also be provided by water convection between eggs or by oxygen production by endogenous algae. We studied egg masses of two local amphibians, Rana sylvatica

Many amphibians rely on wetlands for reproduction and the differential distribution of amphibian species along a gradient of wetland permanence is striking, yet not absolute. Wood frogs (Rana sylvatica) and spotted salamanders (Ambystoma maculatum) are thought to rely on seasonal wetlands

that function relatively poorly in both the pre-and postmetamorphic habitat. We studied predator-mediated selection on tadpoles of the wood frog, Rana sylvatica, to identify this hypothesized period of differential predation risk and estimate its ontogenetic onset. We reared tadpoles in replicated mesocosms

Abstract—Under certain conditions, nitrite can be present in freshwater systems in quantities that are toxic to the fauna. I exposed wood frog (Rana sylvatica) and eastern tiger salamander (Ambystoma tigrinum tigrinum) embryos and young tadpoles and larvae to elevated concentrations of nitrite

License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The wood frog, Rana sylvatica, has numerous adaptations that allow it to survive freezing of up to 65 % of its total body water during the winter. Such adaptations

of osmotic challenge on UT protein and mRNA levels in kidney and urinary bladder of the wood frog (Rana sylvatica), a terrestrial species that tolerates both dehydration and tissue freezing. Using immunoblotting techniques to measure relative UT abundance, we found that UT numbers varied seasonally, with a