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Results 1 - 10
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10,103
Microarray Profiling of Phage-Display Selections for Rapid Mapping of Transcription Factor–DNA Interactions
, 2009
"... Modern computational methods are revealing putative transcription-factor (TF) binding sites at an extraordinary rate. However, the major challenge in studying transcriptional networks is to map these regulatory element predictions to the protein transcription factors that bind them. We have develope ..."
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Cited by 2 (0 self)
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developed a microarray-based profiling of phage-display selection (MaPS) strategy that allows rapid and global survey of an organism’s proteome for sequence-specific interactions with such putative DNA regulatory elements. Application to a variety of known yeast TF binding sites successfully identified
Nanodiscs Allow Phage Display Selection for Ligands to Non-Linear Epitopes on Membrane Proteins
, 2013
"... In this work, we exploited a method that uses polytopic membrane proteins as targets for phage display selections. Membrane proteins represent the largest class of drug targets and drug discovery is mostly based on the identification of ligands binding to target molecules. The screening of a phage d ..."
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In this work, we exploited a method that uses polytopic membrane proteins as targets for phage display selections. Membrane proteins represent the largest class of drug targets and drug discovery is mostly based on the identification of ligands binding to target molecules. The screening of a phage
A "Loop Entropy Reduction" Phage-Display Selection for Folded Amino Acid Sequences
, 2000
"... As a step toward selecting folded proteins from libraries of randomized sequences, we have designed a `loop entropy reduction'-based phage-display method. The basic premise is that insertion of a long disordered sequence into a loop of a host protein will substantially destabilize the host beca ..."
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Cited by 4 (1 self)
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As a step toward selecting folded proteins from libraries of randomized sequences, we have designed a `loop entropy reduction'-based phage-display method. The basic premise is that insertion of a long disordered sequence into a loop of a host protein will substantially destabilize the host
Isolation of a Colon Tumor Specific Binding Peptide Using Phage Display Selection
"... Colorectal cancer is the third most common malig-nancy in the United States. Improved detection sensitivity of early colorectal neoplasms would have important clinical applications. Herein, we report on using phage display to generate peptide libraries that detect colon carcinoma tissues. To accompl ..."
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Cited by 2 (0 self)
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Colorectal cancer is the third most common malig-nancy in the United States. Improved detection sensitivity of early colorectal neoplasms would have important clinical applications. Herein, we report on using phage display to generate peptide libraries that detect colon carcinoma tissues
Specificity improvement of a recombinant anti-testosterone Fab fragment by DCRIII mutagenesis and phage display selection
- Protein Eng
, 1998
"... 1To whom correspondence should be addressed The monoclonal antibodies so far developed by hybridoma technology have not had high enough specificity or affinity to distinguish the closely related steroid hormones in routine clinical assays. We have employed random mutagenesis and phage display approa ..."
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Cited by 3 (0 self)
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1To whom correspondence should be addressed The monoclonal antibodies so far developed by hybridoma technology have not had high enough specificity or affinity to distinguish the closely related steroid hormones in routine clinical assays. We have employed random mutagenesis and phage display
Promoter-targeted Phage Display Selections with Preassembled Synthetic Zinc Finger Libraries for Endogenous Gene
"... Regulation of endogenous gene expression has been achieved using synthetic zinc finger proteins fused to activation or repression domains, zinc finger transcription factors (TFZFs). Two key aspects of selective gene regulation using TFZFs are the accessibility of a zinc finger protein to its target ..."
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-finger zinc finger proteins (ZFPs) displayed on fila-mentous phage, and selected for ZFPs that bound along a 1.4 kb promoter fragment of the human ErbB-2 gene. Following affinity selection by phage display, 13 ZFPs were isolated and sequenced. Transcription factors were prepared by fusion of the zinc finger
Dimerization of a Phage-Display Selected Peptide for Imaging of αvβ6- Integrin: Two Approaches to the Multivalent Effect
"... licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. Received: 2013.10.03; Accepted: 2013.10.10; Published: 2014.05.15 The integrin αvβ6 is an emerging biomarker for non-small cell lung cancer (NSCL ..."
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(NSCLC). An αvβ6-binding peptide was previously selected from a phage-displayed peptide library. Here, we utilize a multivalent design to develop a peptidic probe for positron emission tomography (PET) imaging of αvβ6+ NSCLC tumors. Multimeric presentation of this peptide, RGDLATLRQL, on a bifunctional
Review Phage Display: Selecting Straws Instead of a Needle from a Haystack
, 2011
"... molecules ..."
Results 1 - 10
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10,103
