are thus excluded as a possible source for the increase in oL-subunit mRNA detected by Northern blot analysis. Our results indicate that there is a very specific action of ascorbic acid on the regulation of AChR a-mRNA in the L5 muscle cells, and that the expression of surface receptors in these cells

A weekly 18 spatial resolution optimum interpolation (OI) sea surface temperature (SST) analysis has been produced at the National Oceanic and Atmospheric Administration (NOAA) using both in situ and satellite data from November 1981 to the present. The weekly product has been available since 1993

doi:10.1093/nar/gnh171 Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes

studies show that some miRNAs regulate cell proliferation and apoptosis processes that are important in cancer formation. By using multiple molecular techniques, which include Northern blot analysis, real-time PCR, miRNA microarray, up-or down-expression of specific miRNAs, it was found that several mi

Abstract. Although northern blot analysis is effective for quantifying gene expression, reverse transcription–polymerase chain reaction (RT-PCR) is much more sensitive. Obtaining quantitative RT-PCR results, however, can be challenging. Relative RT-PCR uses standard PCR techniques but permits

that include just the BC forcing 14, 64, 67 show that BC leads to a warming from the surface to about 12 km altitude, by as much 0.6 °C over most of the Northern Hemisphere including the Arctic region (for example, see BC atmospheric heating may be an important contributing factor to the retreat of Himalayan

Figure 4. Northern blot analysis of nacrein. The mantle poly(A)+ RNA (1 g) was separated by electrophoresis through 1 % agarose gel containing formaldehyde and transferred to nitrocellulose filter (Nitroplus 2000, Micron Separation). The filter was hybridized at 65°C in 6 SSC, 5

NTR-1 mRNA could also be used as a specific diagnostic marker and treatment target in pancreatic cancer. Fifteen normal pancreas tissue samples, 20 CP samples, and 30 pancreatic cancer samples were studied. Expression and localization of NTR-1 mRNA was investigated by Northern blot analysis

for the processing of a dozen samples in about 60 minutes, and provides enough RNA to run several northern blots. In our hands methods using glass beads are slower and give lower yields of RNA. RNA was isolated as follows. Ten ml cultures were grown in YPD (1 % yeast extract, 2 % peptone, 2 % dextrose) to an

are homologues of the murlne krox-20 gene. The N-termlnal of the longest cDNA (zf201) contains two acidic regions identical to those of the murlne krox-20. This indicates that the functional organisation of these proteins Is probably conserved. Northern Blot analysis Identified a single transcript of 2.0 kb