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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol 2:2006.0008

by Tomoya Baba, Takeshi Ara, Miki Hasegawa, Yuki Takai, Yoshiko Okumura, Miki Baba, Kirill A Datsenko, Masaru Tomita, Barry L Wanner, Hirotada Mori , 2006
"... We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosom ..."
Abstract - Cited by 714 (7 self) - Add to MetaCart
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation

Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesis

by Friedemann Weber, Anne Bridgen, John K. Fazakerley, Nina Kessler, Richard E. R, M. Elliott, Friedemann Weber, Anne Bridgen, John K. Fazakerley, Hein Streitenfeld, Nina Kessler, Richard E. R, Richard M. Elliott - Proc. Natl. Acad. Sci. USA 98:664 , 2001
"... Updated information and services can be found at: ..."
Abstract - Cited by 70 (26 self) - Add to MetaCart
Updated information and services can be found at:

RESEARCH ARTICLE Statistical Analysis of Hurst Exponents of Essential/Nonessential Genes in 33 Bacterial

by Xiao Liu, Baojin Wang, Luo Xu
"... Methods for identifying essential genes currently depend predominantly on biochemical ex-periments. However, there is demand for improved computational methods for determining gene essentiality. In this study, we used the Hurst exponent, a characteristic parameter to describe long-range correlation ..."
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of the nonessential genes remained un-changed or increased only slightly. All of the Hurst exponents of essential genes followed a normal distribution, with one exception. We therefore propose that the distribution feature of Hurst exponents of essential genes can be used as a classification index for essential gene

Escherichia coli open reading frame 696 is idi, a nonessential gene encoding isopentenyl diphosphate isomerase

by Frederick M. Hahn, Anthony P. Hurlburt, C. Dale Poulter, Isopentenyl Diphosphate Isomerase, A Nonessential Gene Encodingidiis, Gene Encoding, Isopentenyl Diphosphate Isomerase, Frederick M. Hahn, Anthony P. Hurlburt, C. Dale Poulter - J , 1999
"... This article cites 26 articles, 10 of which can be accessed free ..."
Abstract - Cited by 13 (3 self) - Add to MetaCart
This article cites 26 articles, 10 of which can be accessed free

Autographa californica Multiple Nucleopolyhedrovirus me53 (ac140) Is a Nonessential Gene Required for Efficient Budded-Virus Production

by unknown authors , 2008
"... me53 is a highly conserved baculovirus gene found in all lepidopteran baculoviruses that have been fully sequenced to date. The putative ME53 protein contains a zinc finger domain and has been previously described as a major early transcript. We generated an me53-null bacmid (Acme53GFP), as well as ..."
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me53 is a highly conserved baculovirus gene found in all lepidopteran baculoviruses that have been fully sequenced to date. The putative ME53 protein contains a zinc finger domain and has been previously described as a major early transcript. We generated an me53-null bacmid (Acme53GFP), as well

Autographa californica Multiple Nucleopolyhedrovirus me53 (ac140) Is a Nonessential Gene Required for Efficient Budded-Virus Production

by unknown authors , 2008
"... me53 is a highly conserved baculovirus gene found in all lepidopteran baculoviruses that have been fully sequenced to date. The putative ME53 protein contains a zinc finger domain and has been previously described as a major early transcript. We generated an me53-null bacmid (Acme53GFP), as well as ..."
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me53 is a highly conserved baculovirus gene found in all lepidopteran baculoviruses that have been fully sequenced to date. The putative ME53 protein contains a zinc finger domain and has been previously described as a major early transcript. We generated an me53-null bacmid (Acme53GFP), as well

Nonessential genes of phage YeO3-12 include genes involved in adaptation to growth on Yersinia enterocolitica serotype O:3

by Saija Kiljunen , Heikki Vilen , Maria Pajunen , Harri Savilahti , Mikael Skurnik - J. Bacteriol. 187:1405 , 2005
"... ..."
Abstract - Cited by 1 (1 self) - Add to MetaCart
Abstract not found

sequence variants suggest a non-essential gene

by Mohammad M Ghahramani Seno, Benjamin Ym Kwan, Ka Ki, M Lee-ng, Rainald Moessner, Anath C Lionel, Christian R Marshall, Stephen W Scherer
"... Background: Copy number variations (CNVs) can contribute to variable degrees of fitness and/or disease predisposition. Recent studies show that at least 1 % of any given genome is copy number variable when compared to the human reference sequence assembly. Homozygous deletions (or CNV nulls) that ar ..."
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) that are found in the normal population are of particular interest because they may serve to define non-essential genes in human biology. Results: In a genomic screen investigating CNV in Autism Spectrum Disorders (ASDs) we detected a heterozygous deletion on chromosome 10p12.1, spanning the Patched

Replacement Mutagenesis of the Human Cytomegalovirus Genome: US10 and US11 Gene Products Are Nonessential

by J. Virol, T R Jones, V P Muzithras, Y Gluzman, Genecytomegalovirus Genome Us, Thomas R. Jones, Viera P. Muzithras, Yakov Gluzman , 1991
"... products are nonessential. ..."
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products are nonessential.

A Genetic Resource for Rapid and Comprehensive Phenotype Screening of Nonessential Staphylococcus aureus Genes

by unknown authors
"... ABSTRACT To enhance the research capabilities of investigators interested in Staphylococcus aureus, the Nebraska Center for Staphylococcal Research (CSR) has generated a sequence-defined transposonmutant library consisting of 1,952 strains, each containing a single mutation within a nonessential gen ..."
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ABSTRACT To enhance the research capabilities of investigators interested in Staphylococcus aureus, the Nebraska Center for Staphylococcal Research (CSR) has generated a sequence-defined transposonmutant library consisting of 1,952 strains, each containing a single mutation within a nonessential
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