The objective of this study was to elucidate the close similarity in properties between endothelium-derived relaxing factor (EDRF) and nitric oxide radical (NO). Whenever possible, a comparison was also made between arterial and venous EDRF. In vascular relaxation experiments, acetylcholine

of peroxynitrite by a reaction of nitric oxide (NO) and superoxide radical. NO is formed from L-arginine by nitric oxide synthase (NOS). A constitutive cytosolic isoform, neuronal NOS (nNOS), appears to be fairly stable in the postmortem brain tissues. Utilizing a sensitive fluorometrie assay, NO levels were

Nitric oxide (NO) has been implicated in matrix metallopeptidase 9 (MMP9)-dependent mobilization of hematopoietic stem and progenitor cells from bone marrow (BM). However, direct measurement of NO in the BM remained elusive due to its low in situ concentration and short lifetime. Using NO spin

Nitric oxide/Radiosensitivity/Cell cycle/Clonogenic cell survival/Chromosome aberrations. The aim of this study was to examine biological effects of nitric oxide (NO) on radiosensitivity and chromosome aberrations in different phases of the cell cycle in human cancer cells with a wild-type p53 (wtp

was associated with susceptibility to TB. NOS2A-954G/C SNP was not associated with serum levels of nitrite and NO − . These results indicate that variants of IFNG+874T/A SNP and NOS2A-954G/C SNP do not influence TB susceptibility or the secretion of nitric oxide radicals in the study population.

-lates set forth some years ago that free radicals were involved in biologic oxidation-reduction reactions (11, 13). Are some of these free radi-cals OH and/or HO or radicals of a similar high order of reactivity, and where might they arise in the cell? The most likely source of OH and HO, radi-cals

Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Tuberculosis (TB) is one of the most common infectious diseases in the world. Mycobacterium tuberculosis infection leads to pulmonary active disease in approximately 5–10 % of exposed individuals. Both bacteria- and host-related characteristics influence latent infection and disease. Host genetic predisposition to develop TB may involve multiple genes and their polymorphisms. It was reported previously that interferon gamma (IFN-

II. Nitric Oxide 317 A. Discovery of nitric oxide as a biological molecule 317 B. The selective reactivity of nitric oxide 319 C. Diffusion and signaling properties of nitric oxide 319

M of ascorbic acid eqvt/gm of sample). The highest radical scavenging activity by DPPH method was found in methanol extracts (IC 50 587.093µg/ml). Ferrous chelating activity, superoxide radical scavenging activity, nitric oxide radical scavenging activity was found high in acetone, acetone and methanol extracts

Indirect evidence suggests accelerated degradation of endothe-lium-derived nitric oxide (EDNO) by superoxide anion (°2) in hypercholesterolemic vessels (HV). To directly measure 0 2 production by normal vessels (NV) and HV, we used an assay for 02 based on the chemiluminescence (CL) of lucigenin (L