divisions, and c) the ability, although limited, to maintain an undifferentiated state in culture. For these reasons, the use of mouse embryonic stem cells is a fundamental tool for the decipher-ing of the operating mechanisms that need to occur for the in vitro differentiation of insulin-producing cells

type of cells. Results: Mouse embryonic stem cells (ESCs) and mouse embryonic fibroblasts (MEFs) were labeled with QD 655. QDlabeling was monitored by fluorescence microscopy and flow cytometry for 72 hours. Both types of cells were labeled efficiently, but a quick loss of QD-labeling in ESCs

and increases early-stage blastocyst death. Here, we report more detailed examinations of the cytotoxic effects of ginkgolide B on mouse embryonic stem cells (ESCs) and blastocysts and their subsequent development in vitro and in vivo. METHODS AND RESULTS: Using cell culture assay model, we revealed in our

selectable markers in mouse embryonic stem cells.

We have introduced human mini-chromosomes of 4 Mb and ∼15 Mb in size into mouse embryonal stem cells. Although these human mini-chromosomes are stable in hamster and chicken cells, they re-arrange or segregate aberrantly in the embryonal stem cells and are rapidly lost in the absence of selection

CpG island-mediated global gene regulatory

de om) xpansion of ES cells has been branches et al., 2003, 2006, However, in this system, t to monitor and scaling-up s facilitates the expansion of ns while providing a homo-er, for anchorage-dependent essary. A simple method of makes use of microcarriers.

Mouse embryonic stem (ES) cells are endowed with four unusual properties. They are exceedingly small, exhibiting an intra-cellular volume two to three orders of magnitude smaller than that of normal mammalian cells. Their rate of cell division, wherein cell doubling takes place in only 4–5 h

A method for deriving homogenous population of oligodendrocytes from mouse embryonic stem cells

embryonic stem cells. Cotransformation and gene targeting in mouse