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and Cleavable Isotope-Coded Affinity Tag � †
, 2007
"... This article cites 56 articles, 19 of which can be accessed free ..."
Quantitative Protein Profiling Using Two-dimensional Gel Electrophoresis, Isotope-coded Affinity Tag Labeling, and Mass Spectrometry*
"... Quantitative protein profiling is an essential part of pro-teomics and requires new technologies that accurately, reproducibly, and comprehensively identify and quantify the proteins contained in biological samples. We describe a new strategy for quantitative protein profiling that is based on the s ..."
Abstract
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on the separation of proteins labeled with isotope-coded affinity tag reagents by two-dimensional gel elec-trophoresis and their identification and quantification by mass spectrometry. The method is based on the obser-vation that proteins labeled with isotopically different iso-tope-coded affinity tag reagents
Mass Spectrometric Analysis of Protein Mixtures at Low Levels Using Cleavable 13C-Isotope-coded Affinity Tag and
"... In order to identify and compare the protein content of very low quantity samples of high complexity, a protocol has been established that combines the differential profiling strength of a new cleavable 13C isotope-coded affinity tag (cICAT) reagent with the high sequence coverage provided by multid ..."
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In order to identify and compare the protein content of very low quantity samples of high complexity, a protocol has been established that combines the differential profiling strength of a new cleavable 13C isotope-coded affinity tag (cICAT) reagent with the high sequence coverage provided
Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid
"... Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag (ICAT) technology and two-di-mensional liquid c ..."
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Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag (ICAT) technology and two-di-mensional liquid
The Isotope-coded Affinity Tag (ICAT) Method, a Chemical Approach to Quantify Protein Abundance in Complex Proteomes—As
"... are outlined. See text for details. ..."
1Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid Chromatography Mass Spectrometry
"... Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag technology and two-dimensional liquid chromatog ..."
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Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag technology and two-dimensional liquid
Quantitative Protein Profiling Via Isotope-coded Affinity Tag (ICAT) and Tandem Mass Spectrometry I. STATISTICALLY ANNOTATED DATASETS FOR PEPTIDE SEQUENCES AND PROTEINS IDENTIFIED VIA THE
"... Lipid rafts were prepared according to standard protocols from Jurkat T cells stimulated via T cell receptor/CD28 cross-linking and from control (unstimulated) cells. Co-isolating proteins from the control and stimulated cell preparations were labeled with isotopically normal (d0) and heavy (d8) ver ..."
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) versions of the same isotope-coded affinity tag (ICAT) reagent, respectively. Samples were combined, proteolyzed, and resultant peptides fractionated via cat-ion exchange chromatography. Cysteine-containing (ICAT-labeled) peptides were recovered via the biotin tag component of the ICAT reagents by avidin-affinity
Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents: the yeast salinity stress response. Mol. Cell. Proteomics 2:1198–1204
, 2003
"... Protein expression profiles in yeast cells, in response to salinity stress, were determined using the cleavable iso-tope-coded affinity tag (cICAT) labeling strategy. The analysis included separation of the mixed protein samples by SDS-PAGE, followed by excision of the entire gel lane, and division ..."
Abstract
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Cited by 26 (1 self)
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Protein expression profiles in yeast cells, in response to salinity stress, were determined using the cleavable iso-tope-coded affinity tag (cICAT) labeling strategy. The analysis included separation of the mixed protein samples by SDS-PAGE, followed by excision of the entire gel lane, and division
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"... Isotope-coded affinity tag (ICAT) approach to identify and quantify oxidant-sensitive protein thiols ..."
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Isotope-coded affinity tag (ICAT) approach to identify and quantify oxidant-sensitive protein thiols
1Chemical Strategies for Functional Proteomics
"... mass spectrometry; PhIAT, phosphoprotein-specific isotope-coded affinity tag; PMA, 12- ..."
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mass spectrometry; PhIAT, phosphoprotein-specific isotope-coded affinity tag; PMA, 12-
Results 1 - 10
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