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Results 1 - 10
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37
Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents: the yeast salinity stress response. Mol. Cell. Proteomics 2:1198–1204
, 2003
"... Protein expression profiles in yeast cells, in response to salinity stress, were determined using the cleavable iso-tope-coded affinity tag (cICAT) labeling strategy. The analysis included separation of the mixed protein samples by SDS-PAGE, followed by excision of the entire gel lane, and division ..."
Abstract
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Cited by 26 (1 self)
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of the lane into 14 gel regions. Regions were subjected to in-gel digestion, biotin affinity chromatogra-phy, and analysis by nano-scale microcapillary liquid chromatography coupled to tandem mass spectrometry. The novel 13C-labeled ICAT reagents have identical elu-tion profiles for labeled peptide pairs
Multiplexed protein quantitation in Saccharomyces cerevisiae using aminereactive isobaric tagging reagents,”
- Molecular and Cellular Proteomics,
, 2004
"... We describe here a multiplexed protein quantitation strategy that provides relative and absolute measurements of proteins in complex mixtures. At the core of this methodology is a multiplexed set of isobaric reagents that yield amine-derivatized peptides. The derivatized peptides are indistinguisha ..."
Abstract
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Cited by 248 (1 self)
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strategy for reduction of sample complexity (ICAT) is not amenable to identification of post-translationally modified peptides, as the majority of posttranslational modification (PTM) 1 -containing peptides are discarded at the affinity step. We have developed a multiplexed set of reagents for quantitative
ICAT Approach to Redox Proteomics
"... FIG. 1.ICAT approach to redox proteomics. Protein samples with free reactive cysteine thiols and free nonreactive cysteine thiols are exposed to oxidant stress and normal conditions before labeling with ICAT reagents. Some of the reactive cysteine thiols are oxidized depending upon the strength of t ..."
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FIG. 1.ICAT approach to redox proteomics. Protein samples with free reactive cysteine thiols and free nonreactive cysteine thiols are exposed to oxidant stress and normal conditions before labeling with ICAT reagents. Some of the reactive cysteine thiols are oxidized depending upon the strength
Research An Optimized Strategy for ICAT Quantification of Membrane Proteins*
"... The work presented here focuses on the development of a method adapting isotope labeling of proteins with ICAT to the study of highly hydrophobic proteins. Conditions for the labeling of proteins were first established using two standard soluble proteins and iodoacetamidyl-3,6-dioxaoctanediamine bio ..."
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-iodoacetyl biotin and then with the cleavable ICAT reagent. The results presented here show that labeling of proteins with cleavable ICAT is possible and may even be improved in strong denaturing buffers containing both SDS at a concentration higher than 0.5 % (w/v) and urea. These results open the possibility
Quantitative Protein Profiling Via Isotope-coded Affinity Tag (ICAT) and Tandem Mass Spectrometry I. STATISTICALLY ANNOTATED DATASETS FOR PEPTIDE SEQUENCES AND PROTEINS IDENTIFIED VIA THE
"... Lipid rafts were prepared according to standard protocols from Jurkat T cells stimulated via T cell receptor/CD28 cross-linking and from control (unstimulated) cells. Co-isolating proteins from the control and stimulated cell preparations were labeled with isotopically normal (d0) and heavy (d8) ver ..."
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) versions of the same isotope-coded affinity tag (ICAT) reagent, respectively. Samples were combined, proteolyzed, and resultant peptides fractionated via cat-ion exchange chromatography. Cysteine-containing (ICAT-labeled) peptides were recovered via the biotin tag component of the ICAT reagents by avidin
Using ICAT TM Reagent Technology and Current Software Tools to Study Time Course for Protein Release during the Mitochondrial Permeability Transition
"... Isotope Coded Affinity Tags (ICAT TM Reagents) have been used to study global changes in protein expression between control and experimental samples (Scheme 2). The feasibility of using this technique to study subtle changes in relative amounts of protein over time has been evaluated in this work. M ..."
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Isotope Coded Affinity Tags (ICAT TM Reagents) have been used to study global changes in protein expression between control and experimental samples (Scheme 2). The feasibility of using this technique to study subtle changes in relative amounts of protein over time has been evaluated in this work
FOCUS: PROTEOMICS Proteomic Analysis of Pseudomonas aeruginosa Grown Under Magnesium Limitation
"... In this study, large-scale qualitative and quantitative proteomic technology was applied to the analysis of the opportunistic bacterial pathogen Pseudomonas aeruginosa grown under magne-sium limitation, an environmental condition previously shown to induce expression of various virulence factors. Fo ..."
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. For quantitative analysis, whole cell and membrane proteins were differen-tially labeled with isotope-coded affinity tag (ICAT) reagents and ICAT reagent-labeled peptides were separated by two-dimensional chromatography prior to analysis by electros-pray ionization-tandem mass spectrometry (ESI-MS/MS) in an ion
Letter to the Editor PREPARATION OF MONOLITHIC BOROPHOSPHOSIL ICATE GLASS BY THE SOL-GEL METHOD
, 1988
"... A sol-gel procedure is developed to prepare successfully transparent, monolithic and crack-free borophosphosilicate bulk glasses. The reagents of Si(OC2Hs) 4, H3BO 3 and H3PO 4 are used as the starting materials. The maximum total boron and phosphorus contents in borophosphosilicateglasses chieved h ..."
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A sol-gel procedure is developed to prepare successfully transparent, monolithic and crack-free borophosphosilicate bulk glasses. The reagents of Si(OC2Hs) 4, H3BO 3 and H3PO 4 are used as the starting materials. The maximum total boron and phosphorus contents in borophosphosilicateglasses chieved
FOCUS: PROTEOMICS Quantitative Proteomic Analysis of Chromatin-Associated Factors
"... A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncop-rotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extracti ..."
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/salt extraction and analyzed by ICAT reagent labeling, multi-dimen-sional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin
Systematic Uncovering of Multiple Pathways Underlying the Pathology of Huntington Disease by an Acid-cleavable Isotope-coded
"... Huntington disease (HD) is an autosomal dominant neuro-degenerative disease that results from a CAG (glutamine) trinucleotide expansion in exon 1 of huntingtin (Htt). The aggregation of mutant Htt has been implicated in the pro-gression of HD. The earliest degeneration occurs in the striatum. To ide ..."
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, respectively, with the heavy and light forms of the ICAT reagents. Samples were trypsinized, un-covered by avidin affinity chromatography, and analyzed by nano-LC-MS/MS. Western blot analyses were used to con-firm and to calibrate the ICAT ratios. Linear regression was
Results 1 - 10
of
37
