We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation

Chemical probe and missing nucleoside analysis of

... This paper provides an up-to-date critical survey of still- and video-based face recognition research. There are two underlying motivations for us to write this survey paper: the first is to provide an up-to-date review of the existing literature, and the second is to offer some insights

, we suggest that the development of the lateral verbal communication system in man derives from a more ancient communication system based on recognition of hand and face gestures.

... is a benchmark in visual object category recognition and detection, providing the vision and machine learning communities with a standard dataset of images and annotation, and standard evaluation procedures. Organised annually from 2005 to present, the challenge and its associated dataset has

In almost all computer applications, users must enter correct words for the desired objects or actions. For success without extensive training, or in first-tries for new targets, the system must recognize terms that will be chosen spontaneously. We studied spontaneous word choice for objects

that solves the optimization problem in polynomial time for a large class of problems. The proposed method has important applications in areas such as computational biology, natural language processing, information retrieval/extraction, and optical character recognition. Experiments from various domains

be measured more or less straightforwardly at a eiven site, but it is difficult to aggregate these changes regionally and globallv. In contrast to analvses of human al-A11 organisms modify their environment, reasonably well quantified; all are ongoing, teraiion of the global carbon cycle, we and humans

Recent studies of eye movements in reading and other information processing tasks, such as music reading, typing, visual search, and scene perception, are reviewed. The major emphasis of the review is on reading as a specific example of cognitive processing. Basic topics discussed with respect to reading are (a) the characteristics of eye movements, (b) the perceptual span, (c) integration of information across saccades, (d) eye movement control, and (e) individual differences (including dyslexia). Similar topics are discussed with respect to the other tasks examined. The basic theme of the review is that eye movement data reflect moment-to-moment cognitive processes in the various tasks examined. Theoretical and practical considerations concerning the use of eye movement data are also discussed. Many studies using eye movements to investigate cognitive processes have appeared over the past 20 years. In an earlier review, I (Rayner, 1978b) argued that since the mid-1970s we have been in a third era of eye movement research and that the success of research in the current era would depend on the ingenuity of researchers in designing interesting and informative

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