design issues facing the future Internet. In particular, we discuss whether the Internet should adopt a new service model, how this service model should be invoked, and whether this service model should include admission control. These architectural issues are discussed in a nonrigorous manner

Background: A model-based analysis of oligonucleotide expression arrays we developed previously uses a probe-sensitivity index to capture the response characteristic of a specific probe pair and calculates model-based expression indexes (MBEI). MBEI has standard error attached to it as a measure of accuracy. Here we investigate the stability of the probe-sensitivity index across different tissue types, the reproducibility of results in replicate experiments, and the use of MBEI in perfect match (PM)-only arrays. Results: Probe-sensitivity indexes are stable across tissue types. The target gene's presence in many arrays of an array set allows the probe-sensitivity index to be estimated accurately. We extended the model to obtain expression values for PM-only arrays, and found that the 20-probe PM-only model is comparable to the 10-probe PM/MM difference model, in terms of the expression correlations with the original 20-probe PM/MM difference model. MBEI method is able to extend the reliable detection limit of expression to a lower mRNA concentration. The standard errors of MBEI can be used to construct confidence intervals of fold changes, and the lower confidence bound of fold change is a better ranking statistic for filtering genes. We can assign reliability indexes for genes in a specific cluster of interest in hierarchical clustering by resampling clustering trees. A software dChip implementing many of these analysis methods is made available. Conclusions: The model-based approach reduces the variability of low expression estimates, and provides a natural method of calculating expression values for PM-only arrays. The standard errors attached to expression values can be used to assess the reliability of downstream analysis. Published: X Month 2001 Genome Biology...

this paper we discuss the design and implementation of the/'fiesystem interface in the kernel and the NF$ virtual/'fiesystem. We describe some interesting design issues and how they were resolved, and point out some of the shortcomings of the current implementation. We conclude with some

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are sentences of graphical languages. The graphic design issues are codified as expressiveness and effectiveness criteria for graphical languages. Expressiveness criteria determine whether a graphical language can express the desired information. Effectiveness criteria determine whether a graphical language

In this overview paper we motivate the need for and research issues arising from a new model of data processing. In this model, data does not take the form of persistent relations, but rather arrives in multiple, continuous, rapid, time-varying data streams. In addition to reviewing past work

this article, we discuss the fundamentals of distributed DBMS technology. We address the data distribution and architectural design issues as well as the algorithms that need to be implemented to provide the basic DBMS functions such as query processing, concurrency control, reliability

Reliability is even more important in object-oriented programming than elsewhere. This article shows how to reduce bugs by building software components on the basis of carefully designed contracts. 40 s object-oriented techniques steadily gain ground in the world of software development. users

We discuss the design of an acquisitional query processor for data collection in sensor networks. Acquisitional issues are those that pertain to where, when, and how often data is physically acquired (sampled) and delivered to query processing operators. By focusing on the locations and costs

While knowledge is viewed by many as an asset, it is often difficult to locate particular items within a large electronic corpus. This paper presents an agent based framework for the location of resources to resolve a specific query, and considers the associated design issue. Aspects of the work