A role for the MLL fusion partner ENL in transcriptional elongation and

Pluripotent stem cells are capable of differentiating into all cell types of the body and therefore hold tremendous promise for regenerative medicine. Despite their wide-spread use in laboratories across the world, a detailed understanding of the molecular mechanisms that regulate the pluripotent state is currently lacking. Mouse embry-onic (mESC) and epiblast (mEpiSC) stem cells are two closely related classes of pluripotent stem cells, derived from distinct embryonic tissues. Although both mESC and mEpiSC are pluripotent, these cell types show important differences in their properties suggesting distinct pluripo-tent ground states. To understand the molecular basis of pluripotency, we analyzed the nuclear proteomes of mESCs and mEpiSCs to identify protein networks that regulate their respective pluripotent states. Our study

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field of investigation in the search for treatment of human disease is the modulation of chromatin modifications. Chromatin modifications impart virtually all processes occurring in the mammalian nucleus, from regulation of transcription to genomic stability and nuclear high order organization. It has

The surface of nucleosomes is studded with a multiplicity of modifications. At least eight different classes have been characterized to date and many different sites have been identified for each class. Operationally, modifications function either by disrupting chromatin contacts or by affecting

-ious types of stresses. In this study, we focused on the in-volvement of two mutagenic agents, chemical (maleic acid hydrazide; MH) and physical (gamma rays), on the global epigenetic modifications of chromatin in barley. Our results indicate that both mutagens strongly influence the level of histone

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ABSTRACT DNA methylation plays critical roles in gene-silencing through chromatin modification. We reported previously that promoterregion CpG methylation repressed mouse ␦-opioid receptor (mDOR) gene expression. In the current study, we demonstrated that the methylation of mDOR gene promoter

The mechanisms and interplay of coactivators that underlie transcription activation is a critical avenue of investigation in biology today. Using nuclear receptor mediated transcription activation as a model, the nature of coactivator recruitment and chromatin modifications have been found

We have used ChIP-Chip and ChIP-Seq to analyze chromatin modification in MEFs on mouse Chr.17 that contains the imprinted Igf2r gene cluster and applied an algorithm named BLOCs (Broad Local enrichments), that allows the identification of spreading histone modifications. Our results show