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Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics

by Shao-en Ong, Blagoy Blagoev, Irina Kratchmarova, Dan Bach Kristensen, Akhilesh P, Matthias Mann - Mol. Cell. Proteomics , 2002
"... The abbreviations used are: SILAC: Stable isotope labeling by amino acids in cell culture, 2DE: two dimensional (isoelectric focusing/SDS-PAGE) gel electrophoresis: ICATTM: isotope-coded affinity tag; MS: mass spectrometry; MALDI-TOF: matrix assisted laser desorption ionization-time of flight; PMF: ..."
Abstract - Cited by 595 (23 self) - Add to MetaCart
The abbreviations used are: SILAC: Stable isotope labeling by amino acids in cell culture, 2DE: two dimensional (isoelectric focusing/SDS-PAGE) gel electrophoresis: ICATTM: isotope-coded affinity tag; MS: mass spectrometry; MALDI-TOF: matrix assisted laser desorption ionization-time of flight; PMF

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors

by Kazutoshi Yamanaka, Kazutoshi Takahashi, Shinya Yamanaka - Cell
"... posting is granted exceptional permission by the owner of copyright. ..."
Abstract - Cited by 1603 (9 self) - Add to MetaCart
posting is granted exceptional permission by the owner of copyright.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res

by John David Dignam, Russell M. Lebovitz, Robert G. Roeder , 1983
"... We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. T ..."
Abstract - Cited by 1066 (11 self) - Add to MetaCart
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2

CELL-CULTURE-GROWN ORGANISMS

by Lab Anim, J. C. Cox, D. Pye, Information For, J. C. Cox, D. Pye
"... cell-culture-grown organisms Serodiagnosis of nosematosis by immunofluorescence using ..."
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cell-culture-grown organisms Serodiagnosis of nosematosis by immunofluorescence using

Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasm aggregates in cell culture

by Jillian K. Cooper, Gabriele Schilling, Matthew F. Peters, William J. Herring, Alan H. Sharp, Zachary Kaminsky, Jamie Masone, Farhat A. Khan, Michael Delanoy, David R. Borchelt, Valina L. Dawson, Ted M. Dawson, Christopher A. Ross - Hum. Mol. Genet , 1998
"... and cytoplasmic aggregates in cell culture ..."
Abstract - Cited by 34 (1 self) - Add to MetaCart
and cytoplasmic aggregates in cell culture

Cell Culture

by Peter J. Wirth, Stuart H. Yuspa, Snorri S. Thorgeirsson, Et Al, Cancer Res, Contact The Aacr Publications, Peter J. Wirth, Stuart H. Yuspa, Snorri S. Thorgeirsson, Henry Hennings , 2013
"... Terminal differentiation can be induced in cultured basal cells by either increasing the Cai1 * level in the medium from 0.05 to 1.4 mM or by exposure to the tumor promoter 12-O-terradecanoylphorbol-13-acetate (TPA). If ( 'a2 * and TPA act by a common mechanism, then a common pattern of protein ..."
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Terminal differentiation can be induced in cultured basal cells by either increasing the Cai1 * level in the medium from 0.05 to 1.4 mM or by exposure to the tumor promoter 12-O-terradecanoylphorbol-13-acetate (TPA). If ( 'a2 * and TPA act by a common mechanism, then a common pattern

Cell Culture

by Interviews Woo, Suk Hwang, Hans Keirstead, Ron Mckay, Roger Pedersen, Janet Rossant, Michael Rudnicki, Evan Snyder, Alan Trounson, Claude Benchimol, Joe Beechem, John Carrino, David Onions, Brian Pollok
"... www.invitrogen.com FluoCells ® prepared slide #6 showing a fixed, permeabilized, and labeled muntjac skin fibroblast. Mitochondria were labeled with anti-OxPhos Complex V inhibitor protein mouse IgG1 and visualized using orange-fluorescent Alexa Fluor ® 555 goat anti-mouse IgG. F-actin was labeled w ..."
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www.invitrogen.com FluoCells ® prepared slide #6 showing a fixed, permeabilized, and labeled muntjac skin fibroblast. Mitochondria were labeled with anti-OxPhos Complex V inhibitor protein mouse IgG1 and visualized using orange-fluorescent Alexa Fluor ® 555 goat anti-mouse IgG. F-actin was labeled

Cell culture;

by Magdalena Fossum, See Profile, M. Fossum A, J. Svensson A, G. Kratz C, A. Nordenskjöld A , 2006
"... hypospadias repair ..."
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hypospadias repair

Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: down-regulation by cytokines and bacterial products.J. Exp. Mecl

by Federica Sallusto, R Marina Cella, Carlo Danieli, Antonio , 1995
"... We have previously demonstrated that human peripheral blood low density mononuclear cells cultured in granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 develop into dendritic cells (DCs) that are extremely efficient in presenting soluble antigens to T ceils. To identif ..."
Abstract - Cited by 334 (8 self) - Add to MetaCart
We have previously demonstrated that human peripheral blood low density mononuclear cells cultured in granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 develop into dendritic cells (DCs) that are extremely efficient in presenting soluble antigens to T ceils

Limit behaviour of spatially growing cell cultures

by Dirk Hartmann, See Profile, Dirk Hartmann
"... Limit behaviour of spatially growing cell cultures ..."
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Limit behaviour of spatially growing cell cultures
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