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Production and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen
"... Background: Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. T ..."
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. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated recombinant gp63 molecules were studied by an ELISA assay. These two antibodies reacted with the crude soluble antigens prepared from 4 reference strains of Leishmania, 10 isolates from the patients, purified gp63
Absence of Metalloprotease GP63 Alters the Protein Content of
"... Protozoan parasites of Leishmania genus are able to successfully infect their host macrophage due to multiple virulence strategies that result in its deactivation. Recent studies suggest Leishmania GP63 to be a critical virulence factor in modulation of many macrophage molecules, including protein t ..."
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Protozoan parasites of Leishmania genus are able to successfully infect their host macrophage due to multiple virulence strategies that result in its deactivation. Recent studies suggest Leishmania GP63 to be a critical virulence factor in modulation of many macrophage molecules, including protein
BriefDefinitive Report Immunoprotective Zeishmania major Synthetic
"... the structures of a number of gp63 T-cell epitopes which stimulate the proliferation of CD4' cells. One of these synthetic antigens, inoculated subcutaneously with adjuvant, was shown to specifically induce proliferation of the Thl subset and provided immunoprotection against two species of Lei ..."
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of the gp63 T cell epitopes might be predicted and chemically synthesized using the known Leishmania major primary structure (2) and the predictive algorithm proposed by Rothbard and Taylor (3). We report here that three synthetic T cell epitopes of the immunoprotective gp63 molecule stimulate proliferation
Virulent and attenuated lines of Leishmania major : DNA karyotypes and differences in metalloproteinase GP63. Folia Parasitologica 53
, 2006
"... Abstract. The Leishmania metalloproteinase GP63 has been reported to play important roles mainly in resistance of promas-tigotes to complement-mediated lysis and in interaction with macrophage receptors. On the other hand, its function in insect vectors is still unclear. We compared the structure an ..."
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Cited by 1 (0 self)
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of minichromosomes amplified in some lines of the LV561 strain. The results suggest that parasite virulence is not simply correlated with the activity of GP63; however, this enzyme plays a significant role in association with other surface molecules, especially LPG. Overexpression of GP63 can compensate LPG defect
Leishmania-Induced Inactivation of the Macrophage Transcription Factor AP-1 Is Mediated by the Parasite
"... Leishmania parasites have evolved sophisticated mechanisms to subvert macrophage immune responses by altering the host cell signal transduction machinery, including inhibition of JAK/STAT signalling and other transcription factors such as AP-1, CREB and NF-kB. AP-1 regulates pro-inflammatory cytokin ..."
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not block c-Jun down-regulation, suggesting that internalization of the parasite might not be necessary to deliver GP63 molecules inside the host cell. This observation was corroborated by the maintenance of c-Jun cleavage upon incubation with L. mexicana culture supernatant, suggesting that secreted
Reviewed by:
, 2012
"... The intramacrophage protozoan parasites of Leishmania genus have developed sophisticated ways to subvert the innate immune response permitting their infection and propagation within the macrophages of the mammalian host. Several Leishmania virulence factors have been identified and found to be of im ..."
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to be of importance for the development of leishmaniasis. However, recent findings are now further reinforcing the critical role played by the zinc-metalloprotease GP63 as a virulence factor that greatly influence host cell signaling mechanisms and related functions. GP63 has been found to be involved not only
Inhibition of viral replication by monoclonal antibodies directed against human immunodeficiency virus gp120
, 1992
"... Monoclonal antibodies (MAbs) were raised against the glycoprotein gp 120 of human immunodeficiency virus type 1 (strain HTLV-1IIB). The reactivity of five selected MAbs was characterized in several tests: ELISA, immunostaining of Western blots, immuno-fluorescence, immunoprecipitation, immunoelectro ..."
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Cited by 4 (0 self)
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inhibited the spread of HIV-1 infection in cell cultures after a single or repeated treatment at a concentration of 63 ~tg/ml of the purified MAbs. All MAbs showed low but significant neutralizing activity at concentrations of 100 pg/ml. The surface glycoprotein gp 120 of HIV (SU) is proteoly
M (2013) Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. PLoS neglected tropical diseases 7: e2185. doi: 10
- 1371/journal.pntd.0002185 PMID: 23658846 Leishmania Impact on Macrophage Nuclei PLOS Pathogens | DOI:10.1371/journal.ppat.1004776 March 31, 2015 25 / 26
"... Released by many eukaryotic cells, the exosomes are 40–100 nm vesicles shown to operate over the complex processes of cell-cell communication. Among the metazoan cell lineages known to generate exosomes is the mononuclear phagocyte lineage, a lineage that parasites such as Leishmania are known to su ..."
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Cited by 6 (0 self)
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analyses, we found that the macrophage exosomes display unique signatures with respect to composition and abundance of many functional groups of proteins, such as plasma membrane-associated proteins, chaperones and metabolic enzymes. Moreover, for the first time, L. mexicana surface protease GP63 is shown
unknown title
"... Increased platelet adhesion and aggregation has been reported in patients with diabetes mel-litus (1,2). Atherosclerosis occurs in this disease (2,3) and also is the major cause of death in pa-tients with diabetes mellitus, leading to a high mortality in all forms of the disease (4). Platelet-derive ..."
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, and the surface proteins GMP-140(CD62P) and GP-53(CD63) on activated platelets in patients with diabetes and in normal controls to develop a better understanding of their potential contri-bution to diabetic vascular complications. Moreover, we have evaluated the effect of ticlo-pidine on the above
Different selection patterns of resistance and cross-resistance to HIV-1 agents targeting CCR5
"... Objectives: Identification of CCR5 as an antiretroviral target led to the development of several CCR5 antagon-ists in clinical trials and the approval of maraviroc. Evaluating the mechanism of drug resistance to CCR5 agents may have implications in the clinical development of this class of agents. W ..."
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. We have analysed the resistance profile of two R5 HIV-1 strains [BaL and a clinical isolate (CI)] after long-term passage in cell culture in the pres-ence of TAK-779, the first developed non-peptidic small molecule targeting CCR5. Methods: Genotypic and phenotypic tests were used to evaluate
Results 1 - 10
of
18
