Results 11 - 20
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57
A novel 0 thalassemia gene with a single base mutation in the conserved polypyrimidine sequence at the 3 ' end of IVS 2
, 1988
"... An adult Algerian patient with homozygous p thalassemia was found to have a unique p thalasBemia gene. Cloning and sequencing revealed that the only abnormality present in this p gene is a transversion in the polypyrimidine stretch at the 3' end of the large intervening sequence (IVS 2) six bas ..."
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for p thalassemia and carries a haplotype 1 pattern on the other chromosome. The haplotype 1 was shown to be associated with a p ° thalassemia gene due to a previously described one base pair (bp) deletion at codon 6 resulting in a frameshift mutation. The haplotype D associated p gene was cloned
unknown title
, 2007
"... any given PCR reaction may contain PCR chimeras (Cronn et al., 2002) and biases in the success rate for cloning the two haplotypes (Morrell et al., 2006). Also, AS-PCR can fail to prime specifically resulting in genotyping errors. Cloning and AS-PCR are also vulnerable to human and software errors; ..."
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any given PCR reaction may contain PCR chimeras (Cronn et al., 2002) and biases in the success rate for cloning the two haplotypes (Morrell et al., 2006). Also, AS-PCR can fail to prime specifically resulting in genotyping errors. Cloning and AS-PCR are also vulnerable to human and software errors
Structural and Transcriptional Analysis of the Self-Incompatibility Locus of Almond: Identification of a Pollen-Expressed F-Box
"... Gametophytic self-incompatibility in Rosaceae, Solanaceae, and Scrophulariaceae is controlled by the S locus, which consists of an S-RNase gene and an unidentified “pollen S ” gene. An �70-kb segment of the S locus of the rosaceous species almond, the S haplotype–specific region containing the S-RNa ..."
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polymorphism, comparable to that of the S-RNases. The other is unlikely to determine the S specificity of pollen because it showed little allelic sequence polymorphism and was expressed also in pistil. Three other S haplotypes were cloned, and the pollen-expressed genes were physically mapped. In all four
HIGH COMPLEXITY OF PLASMODIUM VIVAX INFECTIONS IN PAPUA NEW
"... Abstract. Although genetically distinct malaria parasites have been shown to simultaneously infect an individual, the total number of unique parasites has not been systematically studied. We examined multiple clones (8–38) from individual blood samples collected from Papua New Guinean children for p ..."
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four-month period. Control polymerase chain reactions (PCRs) detected 0–31 % of clones with haplotypes that arose from PCR artifacts, indicating that caution must be taken when using PCR-based analysis to examine complex infections. To reduce artifacts from clones, analysis was based on haplotypes
, Bermseok Oh
, 2006
"... A human bacterial artificial chromosome (BAC) library was constructed with high molecular weight DNA extracted from the blood of a male Korean. This Korean BAC library contains 100,224 clones of insert size ranging from 70 to 150 kb, with an average size of 86 kb, corresponding to a 2.9-fold redunda ..."
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primer pairs designed for one of the clones. The Korean BAC library and screening pools will allow PCR-based screening of the Korean genome for any gene of interest. We also determined the allele types of HLA-DRA and HLA-DRB3 of clone KB55453, located in the HLA class II region on chromosome 6p21
The Dominance of Alleles Controlling Self-Incompatibility in Brassica Pollen Is Regulated at the RNA Level
"... Self-incompatibility (SI) in Brassica is controlled sporophytically by the multiallelic S-locus. The SI phenotype of pollen in an S-heterozygote is determined by the relationship between the two S-haplotypes it carries, and dominant/recessive relationships often are observed between the two S-haplot ..."
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. The S-locus protein 11 (SP11, also known as the S-locus cysteine-rich protein) gene has been cloned from many pollen-dominant S-haplotypes (class I) and shown to encode the pollen S-determinant. However, SP11 from pollen-recessive S-haplotypes (class II) has never been identified by homology-based
The Dominance of Alleles Controlling Self-Incompatibility in Brassica Pollen Is Regulated at the RNA Level
"... Self-incompatibility (SI) in Brassica is controlled sporophytically by the multiallelic S-locus. The SI phenotype of pollen in an S-heterozygote is determined by the relationship between the two S-haplotypes it carries, and dominant/recessive relationships often are observed between the two S-haplot ..."
Abstract
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. The S-locus protein 11 (SP11, also known as the S-locus cysteine-rich protein) gene has been cloned from many pollen-dominant S-haplotypes (class I) and shown to encode the pollen S-determinant. However, SP11 from pollen-recessive S-haplotypes (class II) has never been identified by homology-based
unknown title
"... Abstract: The Japanese Red Cross Society has appointed representatives to Blood Centers nationwide to collect information on blood transfusion-associated adverse reactions, and data on 310 suspected cases of post-transfusion GVHD were collected during the 8-year period from 1993 to 2000. A diagnosi ..."
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diagnosis of posttransfusion GVHD was made in 61 cases based on the demonstrated presence of donor lymphocytes in the patients' peripheral blood using a microsatellite analysis technique developed by the Japanese Red Cross Society. Case distribution was analyzed according to patient background
unknown title
"... Abstract. Special AT-rich sequence binding protein 1 (SATB1) has regulatory effects on gene expression and appears to play an important role in tumor progression. The present study screened the promoter region of the SATB1 gene for polymorphisms, evaluated the corresponding haplotypes regarding alte ..."
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haplotypes were cloned into the vector pGEM®-T Easy and then transferred into the luc2-containing reporter vector pGl 4.10 ® for transfec-tion of HEK293 cells. The pGl 4.73 ® vector, containing hRluc, was used for normalizing the transfection rates. Sequencing the region-3807 to-2828 bp upstream of ATG from
Temporal Fluctuation of Multidrug Resistant Salmonella Typhi
- Haplotypes in the Mekong River Delta Region of Vietnam. PloS NTD 5: e929
, 2011
"... Background: Typhoid fever remains a public health problem in Vietnam, with a significant burden in the Mekong River delta region. Typhoid fever is caused by the bacterial pathogen Salmonella enterica serovar Typhi (S. Typhi), which is frequently multidrug resistant with reduced susceptibility to flu ..."
Abstract
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Cited by 2 (2 self)
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haplotype H58-C and a localised outbreak of S. Typhi haplotype H58-E2 in 2004. H58-E2-associated typhoid fever cases exhibited evidence of significant geo-spatial clustering along the Sông Hâ u branch of the Mekong River. Multidrug resistance was common in the established clone H58-C
Results 11 - 20
of
57