NCSU Forest Biotechnology Group (2001). Web site hosts NSF Pine Genome Project. URL: http://www2.ncsu.edu/unity/lockers/project/forestbiotech/.  Home/Search   Document Not in Database   Summary   Related Articles

....at NCSU and were cloned from two unrelated genotypes (clones C and D) from the Atlantic Coastal Plain provenance. Choice of Target cDNAs A substantial number of expressed genes (approximately 15,000) have now been identified from loblolly pine as part of the Pine Genome Sequencing Project [NCSU Forest Biotechnology Group, 2001]. The predominant source of these expressed genes is from wood forming tissues. These tissues are rich in expressed genes involved in cell wall biosynthesis and in intracellular signalling. Microarrays have been used with a small subset of these expressed genes to examine gene expression during ....

....genes known to respond to other stresses, such as UV irradiation, pathogen invasion, and sulfur stress (isoflavone reductase like ) Gang et al. 1999] and xenobiotic stress (glutathione S transferases) see Figs. 6 and 7) PCR Amplification 384 ESTs from differentiating xylem or shoot tips [NCSU Forest Biotechnology Group, 2001] with putative functions of interest were selected and PCR amplified using M13 forward and reverse universal primers. PCR was performed in a 50 l reaction containing 39.1 l ddH 2 O, 5 l 10x PCR buffer, 1.5 l MgCl 2 (50mM) 1 l dNTPs (lOmM each) 1 l M13 forward primer (10 M) 1 l M13 reverse ....

NCSU Forest Biotechnology Group (2001). Web site hosts NSF Pine Genome Project. URL: http://www2.ncsu.edu/unity/lockers/project/forestbiotech/.

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