| Fargo, D. C., Zhang, M., Gillham, N. W., Boynton, J. E.: Shine-Dalgarno-like sequences are not required for translation of chloroplast mRNAs in Chlamydomonas reinhardtii chloroplasts or in Escherichia coli. Mol. Gen. Genet. 257: 271-282 (1998). |
....a region of about 7 bases approximately 13 bases upstream of it. While highly variable, this 7 base region is approximately complementary to, and is recognized by binding to the 3 tail of the 16s rRNA. It is known as the Shine Dalgarno (SD) site ( 2] Translation is possible without an SD site([3], 4] but most genes in E. coli have an identifiable SD sequence in the expected location. On the other hand, up to a quarter of E. coli genes do not appear to have one. Based on the 3 tail sequence of the 16s rRNA, the optimum SD sequence is TAAGGAG, and the degree of match is positively ....
Fargo, D. C., Zhang, M., Gillham, N. W., Boynton, J. E.: Shine-Dalgarno-like sequences are not required for translation of chloroplast mRNAs in Chlamydomonas reinhardtii chloroplasts or in Escherichia coli. Mol. Gen. Genet. 257: 271-282 (1998).
....of the genes might be parts of operons, which are multi gene complexes that are transcribed together, possibly not all containing a ribosome binding site. 4. A number of the genes might employ some completely different translation initiation mechanism not involving an SD site. See, for example, Fargo et al. 1998) and Loechel et al. 1991) The last aspect of the computational problem of Section 1.1 to be justified is the fact that instances of the motif will match only inexactly. This is so because the hybridization of the mRNA s SD site and the ribosome s 16S rRNA need only occur with free energy below ....
Fargo, D. C.; Zhang, M.; Gillham, N. W.; and Boynton, J. E. 1998. Shine-Dalgarno-like sequences are not required for translation of chloroplast mRNAs in Chlamydomonas reinhardtii chloroplasts or in Escherichia coli. Molecular and General Genetics 257:271--282.
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